Document Detail


LC determination of aminoglutethimide enantiomers as dansyl and fluorescamine derivatives in tablet formulations.
MedLine Citation:
PMID:  12008127     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Determination of dansyl (AG-DNS) and fluorescamine (AG-F) derivatives of rac-aminoglutethimide in tablet formulation by HPLC has been achieved on a cellulose tris-(3,5-dimethylphenyl carbamate), known as Chiralcel OD and OD-R under normal and reversed phase columns, respectively, using a fluorescence detector (lambda(ex), 360 nm; lambda(em), 530 nm for AG-DNS derivatives; lambda(ex), 395 nm, lambda(em), 495 nm for fluorescamine derivatives (AG-F)). The best results were obtained with mobile phase ethanol:cyclohexane:methanol (95:5:2 v/v/v) for AG-DNS derivatives and acetonitrile:0.5% ortho-phosphoric acid (85:15 v/v) containing 0.26 mM 1-hexanesulfonic acid sodium salt (HSA) for AG-F, respectively. The lower limit of detection (signal to noise ratio of 3:1) were found to be 20 ng ml(-1) for each enantiomer for AG-DNS and 20.5 ng ml(-1) for each diastreoisomer for AG-F.
Authors:
Nesrin Cesur; T Idil Apak; Hassan Y Aboul-Enein; Sumru Ozkirimli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  28     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-05-14     Completed Date:  2002-07-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  487-92     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Istanbul, 34452, Beyazit, Istanbul, Turkey.
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MeSH Terms
Descriptor/Qualifier:
Aminoglutethimide / analysis*
Antineoplastic Agents, Hormonal / analysis*
Cellulose
Chromatography, High Pressure Liquid
Dansyl Compounds / analysis*
Fluorescamine / analogs & derivatives*,  analysis*
Reference Standards
Reproducibility of Results
Solutions
Spectrometry, Fluorescence
Stereoisomerism
Tablets
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Hormonal; 0/Dansyl Compounds; 0/Solutions; 0/Tablets; 125-84-8/Aminoglutethimide; 38183-12-9/Fluorescamine; 9004-34-6/Cellulose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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