Document Detail

[LC-MS/MS analysis of determination of strychnine and brucine in formaldehyde fixed tissue].
MedLine Citation:
PMID:  23213784     Owner:  NLM     Status:  In-Process    
OBJECTIVE: To establish a method for determination of strychnine and brucine in formaldehyde fixed tissue by LC-MS/MS analysis.
METHODS: The samples were pretreated with solid phase extraction using SCX cartridges and separated on SB-C18 column with mobile phase 0.1% formic acid : 0.1% formic acid-acetonitrile (75:25). Electrospray ionization (ESI) source was utilized and operated in positive ion mode. Multiple reactions monitoring (MRM) mode was applied. External standard method was applied for quantitation.
RESULTS: The chromatographic separation of strychnine and brucine in formaldehyde fixed nephritic and hepatic tissues resulted successfully. The standard curve was linear in the range of 0.002-2.0 microg/g for strychnine and brucine in formaldehyde fixed tissues, and the correlation coefficient was more than 0.996. The limits of detection (LOD) of strychnine and brucine in nephritic tissues were 0.06ng/g and 0.03 ng/g, respectively. The LOD of both chemicals were 0.3 ng/g in hepatic tissues. The extraction recovery rate was more than 74.5%. The precision of intra-day and inter-day were both less than 8.2%.
CONCLUSION: Strychnine and brucine can be sensitive to be determined in formaldehyde fixed tissue by LC-MS/MS analysis. It can be applied in the forensic toxicological analysis.
Lan-fen Zhan; Ming-dong Liu; You-yi Yan; Yi Ye; Wei Wang; Zhi-hui Wang; Jun-hong Zhao; Lin-chuan Liao
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Fa yi xue za zhi     Volume:  28     ISSN:  1004-5619     ISO Abbreviation:  Fa Yi Xue Za Zhi     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-12-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426151     Medline TA:  Fa Yi Xue Za Zhi     Country:  China    
Other Details:
Languages:  chi     Pagination:  347-50     Citation Subset:  IM    
West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
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