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LAR inhibition attenuates palmitate induced-insulin resistance in muscle cells.
MedLine Citation:
PMID:  22899729     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Palmitate has been shown to induce insulin resistance in skeletal muscle cells. The aim of this study was to investigate the role of Leukocyte common antigen related (LAR) gene in palmitate-induced insulin resistance in C2C12 cells. A stable C2C12 cell line was generated using LAR short hairpin RNA (shRNA). The levels of LAR protein and phosphorylation of IRS-1 and Akt were detected by western blot analysis. 2-Deoxyglucose uptake was measured in LAR knockdown and control cells using D-[2-3H] glucose. LAR protein level was decreased by 65% in the stable cell line compared to the control cells. 0.5mM palmitate significantly induced LAR mRNA (65%) and protein levels (40%) in myotubes compared to untreated cells. Palmitate significantly reduced insulin-stimulated glucose uptake in both the control and LAR knockdown cells by 33% and 51%, respectively. However, LAR depletion improved insulin-stimulated glucose uptake in myotubes treated with palmitate. Furthermore, the inhibition of LAR prevented palmitate-induced decrease in phosphotylation of IRS-1 Tyr632 and Akt Ser473 in C2C12 cells. In conclusion, these results reveal that palmitate induces LAR expression in C2C12 cells. We also provided the evidence that the inhibition of LAR attenuates palmitate-induced insulin resistance in the myotubes.
Authors:
Sattar Gorgani Firuzjaee; Salar Bakhtiyari; Abolfazl Golestani; Reza Meshkani
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-16
Journal Detail:
Title:  The Journal of endocrinology     Volume:  -     ISSN:  1479-6805     ISO Abbreviation:  J. Endocrinol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
S Gorgani Firuzjaee, Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran, Islamic Republic of.
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