| LAP2alpha-binding protein LINT-25 is a novel chromatin-associated protein involved in cell cycle exit. | |
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MedLine Citation:
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PMID: 17284516 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lamina-associated polypeptide 2alpha (LAP2alpha) is a nuclear protein dynamically associating with chromatin during the cell cycle. In addition, LAP2alpha interacts with A-type lamins and retinoblastoma protein and regulates cell cycle progression via the E2F-Rb pathway. Using yeast two-hybrid analysis and three independent in vitro binding assays we identified a new LAP2alpha interaction partner of hitherto unknown functions, which we termed LINT-25. LINT-25 protein levels were upregulated during G1 phase in proliferating cells and upon cell cycle exit in quiescence, senescence and differentiation. Upon cell cycle exit LINT-25 accumulated in heterochromatin foci, and LAP2alpha protein levels were downregulated by proteasomal degradation. Although LAP2alpha was not required for the upregulation and reorganization of LINT-25 during cell cycle exit, transient expression of LINT-25 in proliferating cells caused loss of LAP2alpha and subsequent cell death. Our data show a role of LINT-25 and LAP2alpha during cell cycle exit, in which LINT-25 acts upstream of LAP2alpha. |
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Authors:
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Nana Naetar; Sabine Hutter; Daniela Dorner; Thomas Dechat; Barbara Korbei; Josef Gotzmann; Hartmut Beug; Roland Foisner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-02-06 |
Journal Detail:
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Title: Journal of cell science Volume: 120 ISSN: 0021-9533 ISO Abbreviation: J. Cell. Sci. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-22 Completed Date: 2007-07-31 Revised Date: 2011-04-06 |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: England |
Other Details:
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Languages: eng Pagination: 737-47 Citation Subset: IM |
Affiliation:
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Max F. Perutz Laboratories, Department of Medical Biochemistry, Medical University of Vienna, Dr. Bohr-Gasse 9, Vienna Biocenter, Dr. Bohr-Gasse 7, A-1030 Vienna, Austria. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Cycle / genetics, physiology Cell Cycle Proteins / genetics, metabolism* Cell Differentiation / drug effects, physiology Cell Line Cells, Cultured Chromatin / metabolism* Chromosomal Proteins, Non-Histone / genetics, metabolism* DNA-Binding Proteins / genetics, metabolism* Gene Expression Regulation Hela Cells Humans Immunoblotting Immunoprecipitation Lamins / metabolism Membrane Proteins / genetics, metabolism* Mice Microscopy, Fluorescence Nuclear Proteins / genetics, metabolism* Protein Binding Reverse Transcriptase Polymerase Chain Reaction Transfection Two-Hybrid System Techniques |
| Grant Support | |
ID/Acronym/Agency:
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P 17871-B09//Austrian Science Fund FWF |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/Chromatin; 0/Chromosomal Proteins, Non-Histone; 0/DNA-Binding Proteins; 0/Lamins; 0/Membrane Proteins; 0/Nuclear Proteins; 0/OIP5 protein, human; 0/lamina-associated polypeptide 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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