| LAP (NF-IL6) transactivates the collagen alpha 1(I) gene from a 5' regulatory region. | |
| | |
MedLine Citation:
|
PMID: 8040336 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Although collagen is known to enhance hepatocyte differentiation and hepatocytes produce collagen in vivo, the transcriptional factors responsible for collagen type I gene expression in hepatic cells are not known. LAP (Liver Activator Protein) is a member of the C/EBP family, which in differentiated hepatocytes contributes to the high levels of liver-specific gene expression. In this study we show that LAP binds to the collagen alpha 1(I) promoter at both reverse CCAAT motifs and activates transcription. Furthermore, an upstream element, collagen element I (-370/-344), which shares homology with the LAP binding cis-element of the albumin promoter (9 of 13 bp) is described. This collagen element I stimulates transcription in both orientations and when placed in front of either a homologous or a heterologous chimeric report construct. These experiments suggest that LAP may be important in the expression of collagen in differentiated hepatocytes through both the promoter and a newly described upstream element. |
| | |
Authors:
|
K Houglum; M Buck; V Adir; M Chojkier |
Related Documents
:
|
20073986 - Expression of runx2 and type x collagen in vertebral growth plate of patients with adol... 16634896 - The expression levels and the differential expression of transforming growth factor-bet... 10367926 - Comparison of growth factor expression in fetal and adult fibroblasts: a preliminary re... 15153536 - Phosphatidylinositol 3-kinase is involved in alpha2(i) collagen gene expression in norm... 9563466 - P53-dependent and -independent regulation of the death receptor killer/dr5 gene express... 15750326 - Aquisition, mobilization and utilization of cellular iron and heme: endless findings an... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of clinical investigation Volume: 94 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1994 Aug |
Date Detail:
|
Created Date: 1994-08-25 Completed Date: 1994-08-25 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 808-14 Citation Subset: AIM; IM |
Affiliation:
|
Department of Medicine, Veterans Affairs Medical Center, San Diego, California. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins Collagen / genetics* DNA / metabolism DNA-Binding Proteins / metabolism, pharmacology* Enhancer Elements, Genetic Genes, Regulator* Humans Molecular Sequence Data Nuclear Proteins / metabolism, pharmacology* Promoter Regions, Genetic Transcription Factors / pharmacology* Transcriptional Activation* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
|
DK 38652/DK/NIDDK NIH HHS; DK 46971/DK/NIDDK NIH HHS; GM 47165/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/CCAAT-Enhancer-Binding Proteins; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/Transcription Factors; 9007-34-5/Collagen; 9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Influenza virus-infected dendritic cells stimulate strong proliferative and cytolytic responses from...
Next Document: FMLP activates Ras and Raf in human neutrophils. Potential role in activation of MAP kinase.