Document Detail

LA-12 overcomes confluence-dependent resistance of HT-29 colon cancer cells to Pt (II) compounds.
MedLine Citation:
PMID:  20530425     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: LA-12 is a new platinum (IV) drug with promising cytotoxic effects in a wide range of cancer cell lines. Its confluence-dependent effects were compared with cisplatin (CDDP) and oxaliplatin (L-OHP) in HT-29 cells. MATERIALS AND METHODS: Cytotoxicity was determined by MTT test, eosin exclusion assay, and cell number quantification. The cell cycle was analysed using propidium iodide DNA staining (flow cytometry), apoptosis by phosphatidylserine externalisation (annexin-V assay), mitochondrial membrane potential by flow cytometry, nuclear morphology by means of fluorescence microscopy, and PARP cleavage by Western blotting. RESULTS: While L-OHP and CDDP were practically inactive in the subconfluent cell population, LA-12 showed a similar toxicity in both subconfluent and growing populations. All compounds induced apoptosis, although with different potentials. CONCLUSION: LA-12 was able to overcome confluence-dependent resistance of HT-29 cells observed for other platinum compounds, which may have potential therapeutic use in slowly growing tumours.
Lenka Svih?lkov?-Sindlerov?; Vendula Foltinov?; Alena Vaculov?; Viktor Horv?th; Karel Soucek; Petr Sova; Jirina Hofmanov?; Alois Kozub?k
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-06-09     Completed Date:  2010-06-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1183-8     Citation Subset:  IM    
Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kr?lovopolsk? 135, 612 65 Brno, Czech Republic.
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MeSH Terms
Adenocarcinoma / drug therapy*
Amantadine / analogs & derivatives*,  pharmacology
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Cell Adhesion / drug effects
Cisplatin / pharmacology
Colonic Neoplasms / drug therapy*,  pathology
Dose-Response Relationship, Drug
Drug Resistance, Neoplasm
HT29 Cells
Organoplatinum Compounds / pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents; 0/Organoplatinum Compounds; 0/bis(acetato)(1-adamantylamine)amminedichloroplatinum(IV); 15663-27-1/Cisplatin; 63121-00-6/oxaliplatin; 768-94-5/Amantadine

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