| LA-12 overcomes confluence-dependent resistance of HT-29 colon cancer cells to Pt (II) compounds. | |
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MedLine Citation:
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PMID: 20530425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: LA-12 is a new platinum (IV) drug with promising cytotoxic effects in a wide range of cancer cell lines. Its confluence-dependent effects were compared with cisplatin (CDDP) and oxaliplatin (L-OHP) in HT-29 cells. MATERIALS AND METHODS: Cytotoxicity was determined by MTT test, eosin exclusion assay, and cell number quantification. The cell cycle was analysed using propidium iodide DNA staining (flow cytometry), apoptosis by phosphatidylserine externalisation (annexin-V assay), mitochondrial membrane potential by flow cytometry, nuclear morphology by means of fluorescence microscopy, and PARP cleavage by Western blotting. RESULTS: While L-OHP and CDDP were practically inactive in the subconfluent cell population, LA-12 showed a similar toxicity in both subconfluent and growing populations. All compounds induced apoptosis, although with different potentials. CONCLUSION: LA-12 was able to overcome confluence-dependent resistance of HT-29 cells observed for other platinum compounds, which may have potential therapeutic use in slowly growing tumours. |
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Authors:
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Lenka Svih?lkov?-Sindlerov?; Vendula Foltinov?; Alena Vaculov?; Viktor Horv?th; Karel Soucek; Petr Sova; Jirina Hofmanov?; Alois Kozub?k |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 30 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-06-09 Completed Date: 2010-06-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 1183-8 Citation Subset: IM |
Affiliation:
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Department of Cytokinetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kr?lovopolsk? 135, 612 65 Brno, Czech Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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drug therapy* Amantadine / analogs & derivatives*, pharmacology Antineoplastic Agents / pharmacology* Apoptosis / drug effects Cell Adhesion / drug effects Cisplatin / pharmacology Colonic Neoplasms / drug therapy*, pathology Dose-Response Relationship, Drug Drug Resistance, Neoplasm HT29 Cells Humans Organoplatinum Compounds / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Organoplatinum Compounds; 0/bis(acetato)(1-adamantylamine)amminedichloroplatinum(IV); 15663-27-1/Cisplatin; 63121-00-6/oxaliplatin; 768-94-5/Amantadine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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