Document Detail


L1 retrotransposition is suppressed by endogenously encoded small interfering RNAs in human cultured cells.
MedLine Citation:
PMID:  16936727     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
LINE-1s, or L1s, are highly abundant retrotransposons comprising 17% of the human genome. Most L1s are retrotransposition defective; nonetheless, there are approximately 100 full-length L1s potentially capable of retrotransposition in the diploid genome. L1 retrotransposition may be detrimental to the host and thus needs to be controlled. Previous studies have identified sense and antisense promoters in the 5' UTR of full-length human L1. Here we show that the resulting bidirectional transcripts can be processed to small interfering RNAs (siRNAs) that suppress retrotransposition by an RNA interference (RNAi) mechanism. We thus provide evidence that RNAi triggered by antisense transcripts may modulate human L1 retrotransposition efficiently and economically. L1-specific siRNAs are among the first natural siRNAs reported in mammalian systems. This work may contribute to understanding the regulatory role of abundant antisense transcripts in eukaryotic genomes.
Authors:
Nuo Yang; Haig H Kazazian
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-08-27
Journal Detail:
Title:  Nature structural & molecular biology     Volume:  13     ISSN:  1545-9993     ISO Abbreviation:  Nat. Struct. Mol. Biol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-06     Completed Date:  2006-10-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101186374     Medline TA:  Nat Struct Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  763-71     Citation Subset:  IM    
Affiliation:
Department of Genetics, University of Pennsylvania School of Medicine, Rm. 475 Clinical Research Building, 415 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
5' Untranslated Regions / genetics
Animals
Base Sequence
Cells, Cultured
Hela Cells
Humans
Mice
Molecular Sequence Data
NIH 3T3 Cells
Promoter Regions, Genetic / genetics
RNA Interference
RNA Stability / genetics
RNA, Small Interfering / genetics,  metabolism*
Retroelements / genetics*
Grant Support
ID/Acronym/Agency:
GM 045398/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/5' Untranslated Regions; 0/RNA, Small Interfering; 0/Retroelements
Comments/Corrections
Comment In:
Nat Struct Mol Biol. 2006 Sep;13(9):758-9   [PMID:  16955095 ]

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