Document Detail

L-tryptophan contaminant 'peak E' induces the release of IL-5 and IL-10 by peripheral blood mononuclear cells from patients with functional somatic syndromes.
MedLine Citation:
PMID:  11703359     Owner:  NLM     Status:  MEDLINE    
In 1989, the development of eosinophilia myalgia syndrome (EMS) was observed in some patients after the intake of l-tryptophan containing several contaminants, including 1,1'-ethylidenebis[l-tryptophan] ('peak E'). Since l-tryptophan has been taken particularly by individuals suffering from functional somatic syndromes (FSS), such as fibromyalgia syndrome (FMS), we put forward the hypothesis that EMS may have developed preferentially in patients with FSS as an allergic reaction towards the contaminant peak E. We therefore studied the immunological reactivity towards l-tryptophan and peak E in these individuals (n = 12) and compared these data with those obtained in 12 healthy controls and 12 patients with other chronic disorders. Peripheral blood mononuclear cells (PBMC) were cultured for 7 days with pure l-tryptophan and peak E. Supernatant fluids were collected at day 7. The type 2 cytokines IL-4, IL-5 and IL-10, and the type 1 cytokines IL-2 and IFN-gamma, were determined by a double sandwich ELISA. PBMC from seven of the 12 FSS patients, but only three of the 24 controls, produced cytokines after incubation with peak E (P < 0.05). Interestingly, six of the seven FSS patients reacting with peak E produced IL-5 and/or IL-10. In contrast, PBMC from only one patient with other chronic disorders and one healthy control secreted type 2 cytokines in response to peak E. The observed heightened type 2 reactivity towards the more immunogenic contaminant 1,1'-ethylidenebis[l-tryptophan] in FSS patients may therefore be taken as an additional argument for our concept that EMS may have developed as a kind of drug-induced allergic disease.
H Barth; R Klein; P A Berg
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  126     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-12     Completed Date:  2001-12-07     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  187-92     Citation Subset:  IM    
Department of Internal Medicine II, University of Tübingen, Germany.
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MeSH Terms
Case-Control Studies
Cytokines / blood
Drug Contamination*
Eosinophilia-Myalgia Syndrome / chemically induced,  etiology,  immunology*
Fibromyalgia / complications,  immunology
Interleukin-10 / blood*
Interleukin-5 / blood*
Leukocytes, Mononuclear / drug effects,  immunology
Middle Aged
Risk Factors
Th2 Cells / drug effects,  immunology
Tryptophan / analogs & derivatives*,  toxicity*
Reg. No./Substance:
0/Cytokines; 0/Interleukin-5; 130068-27-8/Interleukin-10; 132685-02-0/1,1'-ethylidene bis(tryptophan); 73-22-3/Tryptophan

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