| L-lysine transport in chicken jejunal brush border membrane vesicles. | |
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MedLine Citation:
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PMID: 8672084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The properties of l-lysine transport in chicken jejunum have been studied in brush border membrane vesicles isolated from 6-wk-old birds. l-lysine uptake was found to occur within an osmotically active space with significant binding to the membrane. The vesicles can accumulate l-lysine against a concentration gradient, by a membrane potential-sensitive mechanism. The kinetics of l-lysine transport were described by two saturable processes: first, a high affinity-transport system (KmA = 2.4 +/- 0.7 micromol/L) which recognizes cationic and also neutral amino acids with similar affinity in the presence or absence of Na+ (l-methionine inhibition constant KiA, NaSCN = 21.0 +/- 8.7 micromol/L and KSCN = 55.0 +/- 8. 4 micromol/L); second, a low-affinity transport mechanism (KmB = 164. 0 +/- 13.0 micromol/L) which also recognizes neutral amino acids. This latter system shows a higher affinity in the presence of Na+ (KiB for L-methionine, NaSCN = 1.7 +/- 0.3 and KSCN = 3.4 +/- 0.9 mmol/L). L-lysine influx was significantly reduced with N-ethylmaleimide (0.5 mmol/L) treatment. Accelerative exchange of extravesicular labeled l-lysine was demonstrated in vesicles preloaded with 1 mmol/L l-lysine, l-arginine or l-methionine. Results support the view that l-lysine is transported in the chicken jejunum by two transport systems, A and B, with properties similar to those described for systems b0,+ and y+, respectively. |
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Authors:
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M Torras-Llort; R Ferrer; J F Soriano-García; M Moretó |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of membrane biology Volume: 152 ISSN: 0022-2631 ISO Abbreviation: J. Membr. Biol. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1996-09-27 Completed Date: 1996-09-27 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0211301 Medline TA: J Membr Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 183-93 Citation Subset: IM |
Affiliation:
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Unitat de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, E-08028 Barcelona, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine / pharmacology Biological Transport / drug effects Chickens Ethylmaleimide / pharmacology Glucose / metabolism Jejunum / metabolism* Kinetics Lysine / metabolism* Male Methionine / pharmacology Microvilli / drug effects, metabolism* Models, Biological Sodium-Potassium-Exchanging ATPase / metabolism Sucrase Thiocyanates / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Thiocyanates; 128-53-0/Ethylmaleimide; 333-20-0/potassium thiocyanate; 50-99-7/Glucose; 540-72-7/sodium thiocyanate; 56-87-1/Lysine; 63-68-3/Methionine; 74-79-3/Arginine; EC 3.2.1.48/Sucrase; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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