Document Detail

L-lysine transport in chicken jejunal brush border membrane vesicles.
MedLine Citation:
PMID:  8672084     Owner:  NLM     Status:  MEDLINE    
The properties of l-lysine transport in chicken jejunum have been studied in brush border membrane vesicles isolated from 6-wk-old birds. l-lysine uptake was found to occur within an osmotically active space with significant binding to the membrane. The vesicles can accumulate l-lysine against a concentration gradient, by a membrane potential-sensitive mechanism. The kinetics of l-lysine transport were described by two saturable processes: first, a high affinity-transport system (KmA = 2.4 +/- 0.7 micromol/L) which recognizes cationic and also neutral amino acids with similar affinity in the presence or absence of Na+ (l-methionine inhibition constant KiA, NaSCN = 21.0 +/- 8.7 micromol/L and KSCN = 55.0 +/- 8. 4 micromol/L); second, a low-affinity transport mechanism (KmB = 164. 0 +/- 13.0 micromol/L) which also recognizes neutral amino acids. This latter system shows a higher affinity in the presence of Na+ (KiB for L-methionine, NaSCN = 1.7 +/- 0.3 and KSCN = 3.4 +/- 0.9 mmol/L). L-lysine influx was significantly reduced with N-ethylmaleimide (0.5 mmol/L) treatment. Accelerative exchange of extravesicular labeled l-lysine was demonstrated in vesicles preloaded with 1 mmol/L l-lysine, l-arginine or l-methionine. Results support the view that l-lysine is transported in the chicken jejunum by two transport systems, A and B, with properties similar to those described for systems b0,+ and y+, respectively.
M Torras-Llort; R Ferrer; J F Soriano-García; M Moretó
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of membrane biology     Volume:  152     ISSN:  0022-2631     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-09-27     Completed Date:  1996-09-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  183-93     Citation Subset:  IM    
Unitat de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, E-08028 Barcelona, Spain.
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MeSH Terms
Arginine / pharmacology
Biological Transport / drug effects
Ethylmaleimide / pharmacology
Glucose / metabolism
Jejunum / metabolism*
Lysine / metabolism*
Methionine / pharmacology
Microvilli / drug effects,  metabolism*
Models, Biological
Sodium-Potassium-Exchanging ATPase / metabolism
Thiocyanates / pharmacology
Reg. No./Substance:
0/Thiocyanates; 128-53-0/Ethylmaleimide; 333-20-0/potassium thiocyanate; 50-99-7/Glucose; 540-72-7/sodium thiocyanate; 56-87-1/Lysine; 63-68-3/Methionine; 74-79-3/Arginine; EC; EC ATPase

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