| L-lysine production at 50 degrees C by mutants of a newly isolated and characterized methylotrophic Bacillus sp. | |
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MedLine Citation:
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PMID: 2111119 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The amino acid L-lysine was produced from homoserine auxotrophic and S-(2-aminoethyl)-L-cysteine-resistant mutants of a newly isolated gram-positive methylotrophic bacterium, capable of growth on methanol at 60 degrees C. The temperature optimum for growth was between 50 and 53 degrees C. These aerobic, gram-positive, endospore-forming, rod-shaped bacteria required biotin and vitamin B12 for growth. Extracts of the bacteria grown on methanol lacked hydroxypyruvate reductase and contained hexulose 6-phosphate synthase activity. Therefore, these bacteria were considered to be type I methylotrophic bacteria of the genus Bacillus. Fed-batch fermentations resulted in cell densities of 50 g of cell dry weight per liter. Biomass yields on carbon, nitrogen, phosphate, and sulfate were determined. Generation of homoserine auxotrophic and amino acid analog-resistant mutants resulted in L-lysine concentrations of nearly 20 g/liter in fed-batch fermentations. |
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Authors:
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F J Schendel; C E Bremmon; M C Flickinger; M Guettler; R S Hanson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 56 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 1990 Apr |
Date Detail:
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Created Date: 1990-06-19 Completed Date: 1990-06-19 Revised Date: 2010-09-09 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 963-70 Citation Subset: IM |
Affiliation:
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Institute for Advanced Studies in Biological Process Technology, Gortner Laboratories, St. Paul, Minnesota. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus
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genetics,
growth & development,
metabolism* Lysine / biosynthesis* Methanol / metabolism* Mutation Spores, Bacterial / growth & development Temperature |
| Chemical | |
Reg. No./Substance:
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56-87-1/Lysine; 67-56-1/Methanol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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