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L-glutamine provides acid resistance for Escherichia coli through enzymatic release of ammonia.
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PMID:  23337585     Owner:  NLM     Status:  MEDLINE    
Bacteria, exemplified by enteropathogenic Escherichia coli (E. coli), rely on elaborate acid resistance systems to survive acidic environment (such as the stomach). Comprehensive understanding of bacterial acid resistance is important for prevention and clinical treatment. In this study, we report a previously uncharacterized type of acid resistance system in E. coli that relies on L-glutamine (Gln), one of the most abundant food-borne free amino acids. Upon uptake into E. coli, Gln is converted to L-glutamate (Glu) by the acid-activated glutaminase YbaS, with concomitant release of gaseous ammonia. The free ammonia neutralizes proton, resulting in elevated intracellular pH under acidic environment. We show that YbaS and the amino acid antiporter GadC, which exchanges extracellular Gln with intracellular Glu, together constitute an acid resistance system that is sufficient for E. coli survival under extremely acidic environment.
Peilong Lu; Dan Ma; Yuling Chen; Yingying Guo; Guo-Qiang Chen; Haiteng Deng; Yigong Shi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-22
Journal Detail:
Title:  Cell research     Volume:  23     ISSN:  1748-7838     ISO Abbreviation:  Cell Res.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-02     Completed Date:  2013-11-26     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  9425763     Medline TA:  Cell Res     Country:  England    
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Languages:  eng     Pagination:  635-44     Citation Subset:  IM    
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MeSH Terms
Ammonia / metabolism*
Escherichia coli / enzymology,  metabolism*
Escherichia coli Proteins / metabolism*
Glutaminase / metabolism*
Glutamine / metabolism*
Hydrogen-Ion Concentration
Membrane Proteins / metabolism
Reg. No./Substance:
0/Escherichia coli Proteins; 0/Membrane Proteins; 0/xasA protein, E coli; 0RH81L854J/Glutamine; 7664-41-7/Ammonia; EC

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Journal ID (nlm-ta): Cell Res
Journal ID (iso-abbrev): Cell Res
ISSN: 1001-0602
ISSN: 1748-7838
Publisher: Nature Publishing Group
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Copyright © 2013 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Received Day: 04 Month: 12 Year: 2012
Revision Received Day: 12 Month: 12 Year: 2012
Accepted Day: 13 Month: 12 Year: 2012
Print publication date: Month: 05 Year: 2013
Electronic publication date: Day: 22 Month: 01 Year: 2013
pmc-release publication date: Day: 1 Month: 5 Year: 2013
Volume: 23 Issue: 5
First Page: 635 Last Page: 644
PubMed Id: 23337585
ID: 3641589
Publisher Item Identifier: cr201313
DOI: 10.1038/cr.2013.13

L-glutamine provides acid resistance for Escherichia coli through enzymatic release of ammonia Alternate Title:L-glutamine provides acid resistance for E. coli
Peilong Lu123*
Dan Ma123*
Yuling Chen12
Yingying Guo24
Guo-Qiang Chen24
Haiteng Deng12
Yigong Shi123*
1Ministry of Education Key Laboratory of Protein Science, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China
2Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China
3Center for Structural Biology, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China
4Ministry of Education Key Laboratory of Bioinformatics, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China
*These two authors contributed equally to this work.


The food-borne enterohemorrhagic bacteria pose a global health threat. The Escherichia coli(E. coli) strain O157:H7 caused a worldwide pandemic in 19821; whereas the strain O104:H4 was responsible for the European outbreak in 20112, killing 18 and sickening more than 1 500 people. Both to pass through the stomach (pH∼2) and to survive in other acidic environment, E. coli has developed sophisticated acid resistance systems (ARs)3. Of the three known ARs, the functional mechanism of AR1 remains elusive; it requires σS and CRP-activated genes, and can be suppressed by glucose4,5,6,7. By contrast, the molecular mechanisms of AR2 and AR3 have been convincingly deciphered. AR2 comprises an amino acid antiporter GadC8,9, which exchanges extracellular L-glutamate (Glu) with intracellular γ-aminobutyric acid (GABA), and two Glu decarboxylases GadA and GadB10,11, which convert Glu to GABA. Similar to AR2, AR3 also has two components, an L-arginine (Arg)-agmatine antiporter AdiC12,13 and an Arg decarboxylase AdiA14. The net consequence of each complete cycle by AR2 or AR3 is expelling of a cytoplasmic proton to the extracellular environment, thus increasing intracellular pH and consequently promoting survival under acidic environment.

Comprehensive and mechanistic knowledge on bacterial AR is a prerequisite for effective therapeutic intervention. As all enteropathogenic bacteria, mostly food-borne, must pass through the extremely acidic stomach, mechanisms of bacterial survival under environmental pH values of 2-3 are of pivotal significance. At present, our understanding of these mechanisms is far from complete.

GadC was recently found to efficiently transport not only Glu and GABA, but also Gln and L-methionine, in a proteoliposome-based in vitro assay15. For example, the Vmax and Km values for Gln are comparable to, or even better than, those for Glu15. This finding, together with the fact that Gln is an extremely abundant free amino acid in a large variety of food sources (Supplementary information, Table S1), motivated us to investigate whether Gln can be exployed by bacteria such as E. coli to promote survival under acidic environment. In this study, we report the discovery of a new acid resistance system in E. coli, which relies on the glutaminase YbaS and the membrane transporter GadC. This finding is important for understanding bacterial physiology and may offer insights into the antibiotic design for enteropathogenic bacteria.

Acid resistance by L-glutamine

Glu and Arg are known to help bacteria survive the acidic environment, through the actions of AR2 and AR3, respectively3. To systematically examine the roles of other amino acids, we employed a bacterial survival assay7 (Figure 1A). E. coli culture was started in buffered LB medium supplemented with 0.4% glucose, which would suppress AR1, and allowed to grow overnight to reach stationary phase. Then, an aliquot of the culture was diluted 100-fold into the EG medium, pH 2.5, supplemented with one of the 20 natural L-amino acids. After 2 h, cells were checked for viability on LB agar plates.

This result was unequivocal – only two amino acids, Glu and Gln, markedly enhanced the survival of E. coli at pH 2.5 under these experimental conditions (Figure 1B). The survival percentages of E. coli were similar for Glu and Gln. Notably, neither Arg nor any other amino acid provided significant acid resistance in this experimental system. The observation that Arg failed to confer acid resistance is consistent with the knowledge that the Arg decarboxylase AdiA is insufficiently induced in the LB medium16. These results unambiguously demonstrate that Gln alone is sufficient for acid resistance in E. coli.

To examine whether Gln-mediated acid resistance is unique to E. coli, we performed a similar set of experiments in another enteropathogenic bacteria Shigella flexneri (S. flexneri). Consistent with the results of E. coli, survival of S. flexneri under acid shock of pH 2.5 was markedly enhanced by the presence of Gln and Glu, but not by any other amino acid (Supplementary information, Figure S1). These results suggest that Gln might be generally important for acid resistance in bacteria.

Requirement of the glutaminase YbaS for acid resistance

There are at least two potential mechanisms by which Gln may provide acid resistance. One involves decarboxylation, similar to that for Glu by GadA/GadB; and the other involves enzymatic deamidation. Rigorous search of the NCBI database failed to identify any specific Gln decarboxylase in E. coli. In addition, although recombinant GadA or GadB protein efficiently decarboxylates Glu to generate GABA (Supplementary information, Figure S2A-S2F), neither can use Gln as a substrate (Supplementary information, Figure S2G and S2H). These observations effectively rule out decarboxylation as the primary mechanism for Gln-mediated acid resistance. The other possibility – enzymatic deamidation – requires glutaminase. Much to our delight, E. coli contains two glutaminase genes, ybaS and yneH, which are thought to function at acidic and neutral pH ranges, respectively17,18,19 (Figure 2A). We hypothesized that ybaS might be essential for conferring Gln-mediated acid resistance in E. coli.

To examine this hypothesis, we generated two E. coli variants, one with the ybaS gene deleted (ΔybaS) and the other with yneH removed (ΔyneH), and compared their survival to the wild-type (WT) E. coli under acidic environment of pH 2.5. As anticipated, survival of the WT E. coli was greatly facilitated by the presence of either 1 mM Glu or 1 mM Gln (Figure 2B). The variant ΔyneH behaved similarly as the WT E. coli (Figure 2B), suggesting that the glutaminase YneH may not play an important role in acid resistance. In sharp contrast to the WT E. coli and the variant ΔyneH, survival of the ΔybaS variant was only enhanced by the presence of 1 mM Glu, but not by 1 mM Gln (Figure 2B). These experimental results demonstrate that ablation of the ybaS gene abolishes Gln-mediated acid resistance in E. coli, identifying YbaS as the essential glutaminase for acid resistance.

Next, we attempted to rescue survival of the E. coli variant ΔybaS under acidic condition through provision of an exogenous plasmid that expresses the ybaS gene. Under acidic conditions where these E. coli failed to survive in the presence of 1 mM Gln, transformation of the ybaS-containing plasmid, but not the empty plasmid, allowed robust survival (Figure 2C). Expression of the YbaS protein was detected only in cells transformed with the ybaS-containing plasmid, but not with the empty plasmid (Supplementary information, Figure S3, lanes 1 and 2). Together, our experimental evidence demonstrates that the glutaminase YbaS is required for acid resistance in E. coli.

Activation of YbaS at acidic pH

To confirm our genetic observations, we overexpressed ybaS and yneH in E. coli, and biochemically purified the two recombinant proteins to homogeneity (Supplementary information, Figure S4). The glutaminase activities of YbaS and YneH were determined in the pH range of 4.0-8.0 (Figure 2D). Strikingly, YbaS was activated by acidic pH and exhibited robust glutaminase activity only at pH 6.0 or lower. The glutaminase activity of YbaS was the highest at pH 4.0, followed by pH 5.0 and 6.0. At a substrate concentration of 3.3 mM Gln, the glutaminase activity of YbaS at pH 6.6 was ∼3% of that at pH 6.0. At the pH 7.0 and 8.0, YbaS had no detectable activity (Figure 2D). By sharp contrast, YneH displayed high levels of glutaminase activity only at pH 6.0 or higher, with undetectable activity at pH 5.0 or lower (Figure 2D).

The reciprocal glutaminase activities of YbaS and YneH with respect to pH values were further confirmed by their maximal activities (Vmax) (Figure 2E). The Vmax values of YbaS at pH 4.0 and 5.0 are similar to those of YneH at pH 8.0 and 7.0, respectively. Compared to these Vmax values, both YbaS and YneH displayed a reduced level of catalytic activity at pH 6.0 (Figure 2E). The measured glutaminase activities for YbaS and YneH are generally consistent with those reported for E. coli glutaminase A and B, respectively18,19. Thus YbaS and YneH likely correspond to glutaminase A and B, respectively. Together with published reports, our experimental evidence provide a solid biochemical basis for the conclusion that the glutaminase YbaS, but not YneH, is required for Gln-mediated acid resistance in E. coli.

Enzymatic release of ammonium by YbaS

The catalytic activity of glutaminase YbaS should result in generation of the gaseous ammonia. We speculated that some ammonia might be released into the culture medium, perhaps through the action of ammonia transporter or simple membrane permeation20. Under the acidic condition of pH 2.5, the released ammonia should be rapidly protonated into ammonium ion, which has a pKa of 9.25. In this case, we might be able to detect the accumulation of ammonium ion. To examine this scenario, we designed an in vitro assay to continuously measure ammonium concentration (Figure 3A). In this assay, E. coli cells are resuspended in 25 mM citrate buffer, pH 2.5, and the accumulation of ammonium ion is monitored by a specialized ammonium meter.

Supporting our conjecture, the concentration of ammonium ion increased steadily with time for the WT E. coli and the ΔyneH variant in the presence of 1 mM Gln (Figure 3B). By contrast, ammonium concentration remained at the basal level irrespective of time for the E. coli variant ΔybaS (Figure 3B), confirming loss of the glutaminase activity at acidic pH. The temporal increase of ammonium concentration was strictly dependent on the presence of Gln, but not Asn or Glu (Figure 3C; Supplementary information, Figure S5A). In addition, compared to that of 1 mM Gln, the rate of increase of ammonium concentration in the buffer was enhanced by ∼5-fold in the presence of 10 mM Gln (Figure 3B and 3D). Finally, supporting the conclusion of the ybaS-rescue experiment (Figure 2C), provision of the ybaS expression plasmid, but not the empty plasmid, allowed the E. coli variant ΔybaS to release ammonium (Supplementary information, Figure S5B). Together, these results show that the released ammonium is derived from enzymatic activity of the glutaminase YbaS.

Requirement of GadC

For YbaS to mediate acid resistance in E. coli, Gln in the culture medium must be loaded into the cells. Given its known transport activity for Gln15, the amino acid antiporter GadC is likely responsible for the uptake of Gln from the medium and the release of reaction products into the medium. We hypothesized that, under these circumstances, GadC might be required for Gln-mediated acid resistance. To examine this hypothesis, we generated an E. coli variant with the deletion of gadCgadC) and compared its survival to the WT E. coli under the acidic environment of pH 2.5. Loss of the gadC gene not only crippled Glu-mediated acid resistance but also completely abolished the ability of E. coli to resist acidic shock in the presence of Gln (Figure 4A), confirming our prediction. This result further indicates that no other amino acid transporter in E. coli can substitute for GadC in mediating Gln-mediated acid resistance.

We sought to restore acid resistance by transforming the E. coli variant ΔgadC with a plasmid that expresses the GadC protein. Under acidic conditions where these E. coli failed to survive in the presence of Gln, transformation of the gadC-expressing plasmid, but not the empty plasmid, allowed significant survival in the presence of Gln (Figure 4B). Expression of GadC was detected only in E. coli cells transformed with the gadC-expressing plasmid, but not the empty plasmid (Supplementary information, Figure S3, lanes 3 and 4). Finally, the concentrations of released ammonium increased rapidly in the gadC-transformed E. coli, but not in the vector control (Figure 4C). Taken together, these results demonstrate that the transporter GadC is required for Gln- and ybaS-mediated acid resistance in E. coli.

Sufficiency of YbaS-GadC for acid resistance

Upon enzymatic deamidation by YbaS, Gln is converted to Glu, which can be further decarboxylated by GadA or GadB to generate GABA. The latter reaction, as part of AR2, also contributes to acid resistance. To investigate whether the YbaS-GadC system alone is sufficient to confer acid resistance in E. coli, we generated three additional E. coli variants, with deletion of gadA, gadB, or gadA and gadB (abbreviated as gadAB), and compared their survival to the WT E. coli under the acidic environment of pH 2.5 (Figure 5A). As anticipated, deletion of gadA or gadB alone had little impact on the survival of E. coli cells in the presence of either 10 mM Glu or 10 mM Gln. Simultaneous deletion of gadA and gadB, however, crippled the ability of E. coli to survive in the presence of 10 mM Glu, but not 10 mM Gln. The inability to decarboxylate Glu led to abrogation of acid resistance in this E. coli variant in the presence of 10 mM Glu; yet the Gln-mediated acid resistance system, via YbaS and GadC, was still functional. These results indicate that the YbaS-GadC system is sufficient to confer acid resistance in E. coli through YbaS-mediated enzymatic deamidation of Gln and GadC-mediated exchange of extracellular Gln with intracellular Glu. Consistent with this conclusion, ambient ammonium concentrations of the E. coli variant ΔgadAB, but not E. coli ΔybaS, increased steadily under acid shock of pH 2.5 in the presence of Gln (Figure 5B).

To further corroborate our conclusion, we sought to detect and quantify the reaction products of Gln in WT E. coli and the two variants ΔgadAB and ΔybaS, following incubation of these E. coli cells with Gln at pH 2.5. The reaction products from the WT and variant E. coli were differentially labeled with a unique reagent TMT, which is specifically designed for mass spectrometry-based quantification through the formation of a covalent adduct with primary amine. Subsequent mass spectrometric (MS) analysis and MS/MS fragmentation allow quantification of each amino acid and its reaction products. Compared to the Gln-alone control, the combined amounts of Glu and Gln remained largely unchanged for the E. coli variants ΔybaS and ΔgadAB (Supplementary information, Figure S6, upper panel). This result is fully consistent with our prediction. In the case of WT E. coli, Gln and Glu together accounted for ∼80% of the input Gln (Supplementary information, Figure S6, upper panel), suggesting that roughly 20% had been converted to reaction products other than Gln or Glu. Supporting this analysis, GABA was readily detectable by MS/MS fragmentation in the reaction products derived from WT E. coli (Supplementary information, Figure S6, middle panel). In the case of E. coli variant ΔgadAB, GABA was undetectable, yet ∼25% of Gln had been converted to Glu (Supplementary information, Figure S6, lower panel). This result nicely corroborates the conclusion that the YbaS-GadC system alone is sufficient to confer acid resistance in E. coli. Together, the MS analysis is fully consistent with our genetic and biochemical studies (Figures 2,3,4,5).


In this study, we report the identification of a new acid resistance system that relies on the glutaminase YbaS and the amino acid antiporter GadC in E. coli (Figure 5C). Consistent with the need of acid resistance, YbaS (this study) and GadC15 are activated by acidic pH, and only function properly at pH 6.0 or lower. An essential feature of this AR is the requirement of Gln in the acidic environment. Given the abundance of Gln both in natural environment and in food sources (Supplementary information, Table S1), this new AR is likely to have a key role for bacterial survival.

Our experimental evidence suggests that the YbaS-GadC system alone is sufficient for acid resistance, as abrogation of Glu decarboxylation still allowed survival of E. coli under pH 2.5 in the presence of Gln (Figure 5A). We note, however, the percentage of survived E. coli variant ΔgadAB has decreased by ∼50% from that of the WT E. coli (Figure 5A). Accordingly, accumulation of the released ammonium ion in the medium was markedly reduced in E. coli ΔgadAB compared to WT E. coli or E. coli ΔyneH (Supplementary information, Figure S7). This analysis argues that the YbaS-GadC system may work best together with a subsequent decarboxylation step by GadA/GadB (Figure 5C).

Survival of any organism, not just bacteria, must rely on its ability to maintain an adequate pH range. The physiological and biological buffer system is known to rely mainly on ammonia and carbon dioxide21, which are responsible for neutralization of acid (proton) and base (hydroxide ion), respectively. In this regard, biological reactions that involve adsorption or release of NH3/CO2 are likely to play an important role in the regulation of acid-base balance in living organisms. For example, decarboxylation of an amino acid, exemplified by the action of GadA/GadB, results in fixation of a proton, helping to raise intracellular pH. Although only Arg and Glu are currently known to undergo such reactions in E. coli, there is no reason why other amino acids cannot have a similar role in other bacteria and organisms. Along the same line of logic, enzymatic deamidation of Gln and Asn, which releases NH3, should also confer a means of acid resistance. In this study, we discovered the Gln-mediated acid resistance system. It is tempting to speculate that Asn-related system might function in some other bacteria or organisms. Notably, release of NH3 for acid resistance, as demonstrated in this study for E. coli, has been previously demonstrated in the bacterium Helicobacter pylori, which fights gastric acid through enzymatic breakdown of urea by urease22,23. Notably, a variety of bacterial species utilize the arginine deiminase system to help fight acid stress, which converts arginine to ornithine, ammonia and carbon dioxide24. Similar mechanisms might be much more widespread than reported to date.

Materials and Methods
Bacterial strain and culture condition

Most experiments reported in this manuscript were performed using the E. coli strain MG1655, which has been widely used in the study of acid resistance by E. coli5,12. Deletion of specific gene(s) in this E. coli strain was generated using the one-step gene inactivation technique25. To create rescue plasmids, E. coligadC and ybaS genes were subcloned into the pQLINK vector26, each with a His6 tag at the N-terminus. The expression levels of GadC and YbaS were monitored by western blot using a monoclonal antibody against the His6 tag. Control experiments were performed with cells transformed with the empty pQLINK vector. In addition, the Gram-negative bacterium S. flexneri was purchased from China General Microbiological Culture Collection (CGMCC). The minimal medium used was E medium supplemented with 0.5% glucose (known as the EG medium27). E medium contains 73 mM K2HPO4, 17 mM NaNH4HPO4, 0.8 mM MgSO4 and 10 mM citrate. The EG medium was adjusted to pH 2.5 using hydrochloric acid (HCl) and sterile filtered. The complex medium used was Luria-Bertani (LB) broth, supplemented with 0.4% glucose and 100 mM morpholinepropanesulfonic acid buffer (MOPS, pH 8.0). The complex medium is known as the LBG medium.

Acid resistance assay

The acid resistance system was assayed after overnight (20 h) growth in the LBG medium. The overnight culture was diluted 1:100 into EG medium, pH 2.5, and incubated at 37 °C for 2 h. Then the cells were diluted in LB medium, plated to LB agar, and incubated at 37 °C for 15 h before colony count. The numbers of colony serve as the numerators for calculation of percent E. coli survival. In the mean time, the overnight culture without acid shock was diluted in LB medium, plated on LB agar, and incubated at 37 °C for 15 h; the number of colony serves as the denominator for calculation of percent E. coli survival. The E. coli cell densities of the overnight culture in different experiments were approximately the same at 5 × 109/ml. Survival values reported are the averages of three independent experiments.

To assay the role of individual amino acid, overnight E. coli culture was diluted 1:100 into EG medium, pH 2.5, supplemented with one of the 20 natural L-amino acids at 1 mM. The negative control for these survival experiments involved an acid challenge (pH 2.5) in EG medium without amino acid supplement.

Protein preparation

The cDNAs of the full-length gadA, gadB, yneH and ybaS genes were individually cloned into pET15b (Novagen). Overexpression of the recombinant protein was induced by 0.2 mM isopropyl β-d-thiogalactoside (IPTG) when the cells reached an optical density value of 1.2 at 600 nm. After growth at 18 °C for 12 h, the cells were collected, homogenized in a buffer containing 25 mM Tris-HCl, pH 8.0, and 150 mM NaCl. After sonication, cell debris was removed by low-speed centrifugation for 1 h. The supernatant was collected and loaded onto Ni2+-nitrilotriacetate affinity resin (Ni-NTA, Qiagen), followed by a wash with 25 mM Tris-HCl, pH 8.0, 150 mM NaCl and 20 mM imidazole. The target protein was eluted by an elution buffer containing 250 mM imidazole, 25 mM Tris, pH 8.0 and 150 mM NaCl, and further purified by anion exchange chromatography (Source-15Q, GE Healthcare). The protein was further purified by gel filtration (Superdex-200, 10/30, GE Healthcare) with a buffer containing 150 mM NaCl and 25 mM Tris, pH 8.0.

Glutaminase activity assay

The initiation solution, containing 0.1 ml of freshly made Gln solution at desired concentration, 10 μl of 1 M buffer solution and 37.5 μl H2O, was equilibrated at 37 °C. The reaction was started by adding 2.5 μl of 0.1 mg/ml protein (YbaS or YneH) to the initiation solution and allowed to continue at 37 °C for 1 min. The reaction was stopped by boiling for 10 min. A 50-μl aliquot of the supernatant was added to a reaction mixture containing 0.5 ml of hydroxylamine buffer (0.25 M hydroxylamine and 20 mM EDTA, pH 8.0), 25 μl of 100 mM NAD+ solution, 0.425 ml of distilled water and 5 units of bovine Glu dehydrogenase (Sigma-Aldrich). After incubation at 37 °C for 1 h, the optical density at 340 nm was measured. For determination of Vmax and Km at different pH values, six serial dilutions of Gln were made to achieve a range of concentrations from 50 mM to 1.67 mM. Measurements were done in triplicates and analyzed using GRAPHPAD PRISM 5.0 Demo (GraphPad Software, San Diego, CA, USA).

Decarboxylation reaction for MS analysis

Solution A contained 10 μg GadA or GadB, 10 mM citrate, pH 4.5, and 0.5 mM pyridoxal 5′-phosphate (PLP). Solution B contained 20 mM Glu or Gln, 10 mM citrate, pH 4.5, and 0.5 mM PLP. Both solutions A and B were individually equilibrated at 37 °C. The reaction was started by mixing 100 μl solution A with 100 μl solution B and allowed to proceed at 37 °C for 10 min. Reaction was stopped by flash-freeze with liquid nitrogen, and samples were subjected to MS detection.

Ammonium release assay

The acid resistance system was assayed after overnight (20 h) growth in LB broth buffered to pH 8.0 with 100 mM MOPS and supplemented with 0.4% glucose (LBG medium). The initial cell density was measured, and adjusted to the same OD600 value. One ml of cell culture was centrifuged. The pellet was washed with distilled water, pelleted again and resuspended in distilled water before mixing with 10 ml citrate buffer, pH 2.5, supplemented with a specific amino acid. The ammonium concentration was continuously measured by the DUAL STAR Ammonium Ion Selective Electrode Meter (Thermo Scientific Orion).

Mass spectrometry

For detection and quantification of Gln/Gln and their enzymatic reaction products, samples were labeled with a series of TMT reagents (Thermo, Pierce Biotechnology, Rockford, IL, USA) according to the manufacturer's instruction. Each TMT reagent is an isobaric tag, comprising an amine-reactive NHS-ester group, a spacer arm, and an MS/MS reporter28,29. Each sample is labeled with a unique TMT reagent, which gives rise to a unique reporter mass during fragmentation in the MS/MS spectrum. These reporter ions, in the low mass region of the MS/MS spectrum, are used to report relative concentrations of the target molecules in distinct samples. Each TMT reagent, out of a set of six reagents in total, has the same nominal parent (precursor) mass, and the labeled molecules from different samples also have the same mass; however, the masses differ in the balancing and reporter region, and are detected in the MS/MS spectra.

Briefly, the TMT reagents were dissolved by anhydrous acetonitrile. The labeling reaction was carried out by incubation of Gln/Glu and their derivatives with the TMT reagents for 1 h at room temperature, and the reaction was quenched using hydroxylamine. The TMT-labeled Gln/Glu and their derivatives were analyzed by LC-MS/MS. The TMT-labeled products were separated by a 65-min gradient elution at a flow rate 0.25 μl/min with the EASY-nLCII- integrated nano-HPLC system (Proxeon, Denmark), which is directly interfaced with the Thermo LTQ-Orbitrap mass spectrometer. The analytical column was a home-made fused silica capillary column (75 μm ID, 150 mm length; Upchurch, Oak Harbor, WA, USA) packed with C-18 resin (300 A, 5 μm, Varian, Lexington, MA, USA). Mobile phase A consisted of 0.1% formic acid, and mobile phase B consisted of 100% acetonitrile and 0.1% formic acid. The LTQ-Orbitrap mass spectrometer was operated in the data-dependent acquisition mode using the Xcalibur 2.0.7 software, and there is a single full-scan mass spectrum in the Orbitrap (100-1 000 m/z, 30 000 resolution) followed by three MS/MS scans in the quadruple collision cell using the higher energy collision dissociation (HCD), in which the precursor mass selection window was set as 2 Da. The MS/MS spectra from each LC-MS/MS run were analyzed to find the labeled Gln/Glu and their corresponding fragments.


(Supplementary information is linked to the online version of the paper on the Cell Research website.)

This work was supported by funds from the Ministry of Science and Technology (2009CB918801), the National Natural Science Foundation of China (31130002, 31021002, and 30888001), and the Beijing Municipal Commissions of Education and Science and Technology.

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Supplementary Material Supplementary information, Table S1

Content of free L-amino acids in various food sources (mg/100g)

Supplementary information, Figure S1

L-glutamine markedly enhances survival of the Gram-negative bacterium S. flexneri at pH 2.5.

Supplementary information, Figure S2

Neither GadA nor GadB is able to use Gln as the substrate for α-decarboxylation.

Supplementary information, Figure S3

Confirmation of expression of GadC and YbaS in the rescue experiments.

Supplementary information, Figure S4

Purification of recombinant glutaminases YbaS and YneH.

Supplementary information, Figure S5

Measurement of ammonium accumulation in citrate buffer (pH 2.5).

Supplementary information, Figure S6

Mass spectrometric analysis of Gln and its reaction products at pH 2.5 by WT E. coli and its variants.

Supplementary information, Figure S7

Release of ammonium ion by WT E. coli and its variants.

Article Categories:
  • Original Article

Keywords: acid resistance, bacterial stress response, glutaminase, glutamine, amino acid antiporter.

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