Document Detail

L-asparaginase II of saccharomyces cerevisiae. Activity profile during growth using an ure2 mutant P40-3C and a P40-3C + URE2p strain.
MedLine Citation:
PMID:  10399275     Owner:  NLM     Status:  MEDLINE    
The activity profile of the periplasmic asparaginase of Saccharomyces cerevisiae was determined during cell growth in an ure2 mutant; in an ure2 transformed with a plasmid containing the gene URE2 and, for comparison, in the strain D273-10B. Cells were cultivated in media presenting variable quantitative and qualitative nitrogen availability and the enzyme activity was evaluated in fresh and in nitrogen-starved cells. Nitrogen affected the asparaginase II level in fresh and starved cells of all strains. In the best condition, enzyme was produced by the wild-type cells at the late log-phase in the glucose/ammonium medium with a carbon to nitrogen ratio 4.3:1. Upon starvation, the activity doubled. The overall profile of the transformed strain was similar to that of the wild-type strain. In the ure2 mutant, high-enzyme levels were observed during growth, as expected. However the activity level, upon starvation, in proline grown cells, increased sixfold, suggesting that in addition to the Ure2p-Gln3p system, another system regulates asparaginase II biosynthesis.
E M Oliveira; E Carvajal; E P Bon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  77-79     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  1999  
Date Detail:
Created Date:  1999-08-24     Completed Date:  1999-08-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  311-6     Citation Subset:  IM    
Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Brazil.
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MeSH Terms
Asparaginase / metabolism*
Fungal Proteins / genetics*
Glutathione Peroxidase
Nitrogen / metabolism
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae Proteins*
Reg. No./Substance:
0/Fungal Proteins; 0/Prions; 0/Saccharomyces cerevisiae Proteins; 7727-37-9/Nitrogen; EC Peroxidase; EC protein, S cerevisiae; EC

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