| L-asparaginase II of saccharomyces cerevisiae. Activity profile during growth using an ure2 mutant P40-3C and a P40-3C + URE2p strain. | |
| | |
MedLine Citation:
|
PMID: 10399275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The activity profile of the periplasmic asparaginase of Saccharomyces cerevisiae was determined during cell growth in an ure2 mutant; in an ure2 transformed with a plasmid containing the gene URE2 and, for comparison, in the strain D273-10B. Cells were cultivated in media presenting variable quantitative and qualitative nitrogen availability and the enzyme activity was evaluated in fresh and in nitrogen-starved cells. Nitrogen affected the asparaginase II level in fresh and starved cells of all strains. In the best condition, enzyme was produced by the wild-type cells at the late log-phase in the glucose/ammonium medium with a carbon to nitrogen ratio 4.3:1. Upon starvation, the activity doubled. The overall profile of the transformed strain was similar to that of the wild-type strain. In the ure2 mutant, high-enzyme levels were observed during growth, as expected. However the activity level, upon starvation, in proline grown cells, increased sixfold, suggesting that in addition to the Ure2p-Gln3p system, another system regulates asparaginase II biosynthesis. |
| | |
Authors:
|
E M Oliveira; E Carvajal; E P Bon |
Related Documents
:
|
22043305 - Up-regulation of ndrg2 in senescent lens epithelial cells contributes to age-related ca... 1443165 - Pathways for glucose transport in type ii pneumocytes freshly isolated from adult guine... 10948345 - Antitumoral action of 2-deoxy-d-glucose tetraacetate in human melanoma cells. 20518065 - Flow-regulated glucose and lipid metabolism in adipose tissue, endothelial cell and hep... 10747035 - Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase a... 11720305 - Enhancement of beta-sitosterol transformation in mycobacterium vaccae with increased ce... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Applied biochemistry and biotechnology Volume: 77-79 ISSN: 0273-2289 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 1999 |
Date Detail:
|
Created Date: 1999-08-24 Completed Date: 1999-08-24 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 311-6 Citation Subset: IM |
Affiliation:
|
Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Brazil. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Asparaginase
/
metabolism* Fungal Proteins / genetics* Genotype Glutathione Peroxidase Nitrogen / metabolism Prions* Saccharomyces cerevisiae / enzymology* Saccharomyces cerevisiae Proteins* |
| Chemical | |
Reg. No./Substance:
|
0/Fungal Proteins; 0/Prions; 0/Saccharomyces cerevisiae Proteins; 7727-37-9/Nitrogen; EC 1.11.1.9/Glutathione Peroxidase; EC 1.11.1.9/URE2 protein, S cerevisiae; EC 3.5.1.1/Asparaginase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A high-copy-number plasmid capable of replication in thermophilic cyanobacteria.
Next Document: Bioconversion of secondary fiber fines to ethanol using counter-current enzymatic saccharification a...