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L-arginine or tempol supplementation improve renal and cardiovascular function in rats with reduced renal mass and chronic high salt intake.
MedLine Citation:
PMID:  23387940     Owner:  NLM     Status:  Publisher    
AIM: Early life reduction in nephron number and chronic high salt intake cause development of renal and cardiovascular disease, which has been associated with oxidative stress and nitric oxide (NO) deficiency. We investigated the hypothesis that interventions stimulating NO signalling or reducing oxidative stress, may restore renal autoregulation, attenuate hypertension, and reduce renal and cardiovascular injuries following reduction in renal mass reduction and chronic high salt intake. METHODS: Male Sprague-Dawley rats were uninephrectomized (UNX) or sham-operated at 3-weeks of age and given either a normal-salt (NS) or high-salt (HS) diet. Effects on renal and cardiovascular functions were assessed in rats supplemented with substrate for NO synthase (L-Arg) or a superoxide dismutase mimetic (tempol). RESULTS: Rats with UNX+HS developed hypertension and displayed increased renal NADPH oxidase activity, elevated levels of oxidative stress markers in plasma and urine, and reduced cGMP in plasma. Histological analysis showed signs of cardiac and renal inflammation and fibrosis. These changes were linked with abnormal renal autoregulation, measured as a stronger tubuloglomerular feedback (TGF) response. Simultaneous treatment with L-Arg or tempol restored cGMP levels in plasma and increased markers of NO signalling in the kidney. This was associated with normalized TGF responses, attenuated hypertension, and reduced signs of histopathological changes in the kidney and in the heart. CONCLUSION: Reduction in nephron number during early life followed by chronic HS intake is associated with oxidative stress, impaired renal autoregulation and development of hypertension. Treatment strategies that increase NO bioavailability, or reduce levels of reactive oxygen species, were proven beneficial in this model of renal and cardiovascular disease. Acta Physiologica © 2013 Scandinavian Physiological Society.
Mattias Carlström; Russell D Brown; Ting Yang; Michael Hezel; Erik Larsson; Peter G Scheffer; Tom Teerlink; Jon O Lundberg; A Erik G Persson
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-7
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  -     ISSN:  1748-1716     ISO Abbreviation:  Acta Physiol (Oxf)     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Acta Physiologica © 2013 Scandinavian Physiological Society.
Department of Physiology and Pharmacology, Karolinska Institutet, Sweden; Department of Medical Cell Biology, Uppsala University, Sweden.
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