Document Detail

L-arginine protects human heart cells from low-volume anoxia and reoxygenation.
MedLine Citation:
PMID:  11834473     Owner:  NLM     Status:  MEDLINE    
Protective effects of L-arginine were evaluated in a human ventricular heart cell model of low-volume anoxia and reoxygenation independent of alternate cell types. Cell cultures were subjected to 90 min of low-volume anoxia and 30 min of reoxygenation. L-Arginine (0-5.0 mM) was administered during the preanoxic period or the reoxygenation phase. Nitric oxide (NO) production, NO synthase (NOS) activity, cGMP levels, and cellular injury were assessed. To evaluate the effects of the L-arginine on cell signaling, the effects of the NOS antagonist N(G)-nitro-L-arginine methyl ester, NO donor S-nitroso-N-acetyl-penicillamine, guanylate cyclase inhibitor methylene blue, cGMP analog 8-bromo-cGMP, and ATP-sensitive K+ channel antagonist glibenclamide were examined. Our data indicate that low-volume anoxia and reoxygenation increased NOS activity and facilitated the conversion of L-arginine to NO, which provided protection against cellular injury in a dose-dependent fashion. In addition, L-arginine cardioprotection was achieved by the activation of guanylate cyclase, leading to increased cGMP levels in human heart cells. This action involves a glibenclamide-sensitive, NO-cGMP-dependent pathway.
Noritsugu Shiono; Vivek Rao; Richard D Weisel; Muneyasu Kawasaki; Ren-Ke Li; Donald A G Mickle; Paul W M Fedak; Laura C Tumiati; Lawrence Ko; Subodh Verma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  282     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-02-08     Completed Date:  2002-04-03     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H805-15     Citation Subset:  IM    
Division of Cardiac Surgery, University of Toronto, Toronto, Ontario, M5G 2C4 Canada.
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MeSH Terms
Arginine / pharmacology*
Cell Hypoxia / drug effects*
Cells, Cultured
Heart / drug effects,  physiology*
Models, Cardiovascular
Myocardium / cytology,  enzymology
Nitric Oxide Synthase / metabolism
Nitric Oxide Synthase Type II
Nitric Oxide Synthase Type III
Reg. No./Substance:
74-79-3/Arginine; EC protein, human; EC protein, human; EC Oxide Synthase; EC Oxide Synthase Type II; EC Oxide Synthase Type III

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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