| L-arginine protects human heart cells from low-volume anoxia and reoxygenation. | |
MedLine Citation:
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PMID: 11834473 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Protective effects of L-arginine were evaluated in a human ventricular heart cell model of low-volume anoxia and reoxygenation independent of alternate cell types. Cell cultures were subjected to 90 min of low-volume anoxia and 30 min of reoxygenation. L-Arginine (0-5.0 mM) was administered during the preanoxic period or the reoxygenation phase. Nitric oxide (NO) production, NO synthase (NOS) activity, cGMP levels, and cellular injury were assessed. To evaluate the effects of the L-arginine on cell signaling, the effects of the NOS antagonist N(G)-nitro-L-arginine methyl ester, NO donor S-nitroso-N-acetyl-penicillamine, guanylate cyclase inhibitor methylene blue, cGMP analog 8-bromo-cGMP, and ATP-sensitive K+ channel antagonist glibenclamide were examined. Our data indicate that low-volume anoxia and reoxygenation increased NOS activity and facilitated the conversion of L-arginine to NO, which provided protection against cellular injury in a dose-dependent fashion. In addition, L-arginine cardioprotection was achieved by the activation of guanylate cyclase, leading to increased cGMP levels in human heart cells. This action involves a glibenclamide-sensitive, NO-cGMP-dependent pathway. |
Authors:
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Noritsugu Shiono; Vivek Rao; Richard D Weisel; Muneyasu Kawasaki; Ren-Ke Li; Donald A G Mickle; Paul W M Fedak; Laura C Tumiati; Lawrence Ko; Subodh Verma |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 282 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-02-08 Completed Date: 2002-04-03 Revised Date: 2011-10-27 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H805-15 Citation Subset: IM |
Affiliation:
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Division of Cardiac Surgery, University of Toronto, Toronto, Ontario, M5G 2C4 Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arginine
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pharmacology* Cell Hypoxia / drug effects* Cells, Cultured Heart / drug effects, physiology* Humans Immunohistochemistry Models, Cardiovascular Myocardium / cytology, enzymology Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type II Nitric Oxide Synthase Type III Oxidation-Reduction |
| Chemical | |
Reg. No./Substance:
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74-79-3/Arginine; EC 1.14.13.39/NOS2 protein, human; EC 1.14.13.39/NOS3 protein, human; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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