Document Detail


L-amino acid oxidase activity present in fractions of Bothrops jararaca venom is responsible for the induction of programmed cell death in Trypanosoma cruzi.
MedLine Citation:
PMID:  20615423     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bothrops jararaca venom induces programmed cell death in epimastigotes of Trypanosoma cruzi. Here we fractionated the venom and observed that the anti-T. cruzi activity was associated with fractions that present L-amino acid oxidase (L-AAO) activity. L-AAO produces H(2)O(2), which is highly toxic. The addition of catalase to the medium, a H(2)O(2) scavenger, reverted the killing capacity of venom fractions. The anti-T. cruzi activity was also abolished when parasites were cultured in a medium without hydrophobic amino acids that are essential for L-AAO activity. These results were confirmed with a commercial purified L-AAO. Treatment for 24 h with fractions that present L-AAO activity induced parasites cytoplasmic retraction, mitochondrial swelling and DNA fragmentation, all morphological characteristics of programmed cell death. Similar changes were also observed when parasites were treated with H(2)O(2). These results indicate that H(2)O(2), the product of L-AAO reaction, induces programmed cell death explaining the anti-T. cruzi activity of B. jararaca venom.
Authors:
Poliana Deolindo; André S Teixeira-Ferreira; Renato A DaMatta; Elias W Alves
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-06
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  56     ISSN:  1879-3150     ISO Abbreviation:  Toxicon     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-08-30     Completed Date:  2010-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  944-55     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, 28013-600 Campos dos Goytacazes, RJ, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antiprotozoal Agents / isolation & purification,  pharmacology*
Apoptosis / drug effects*
Bothrops / physiology*
Catalase / metabolism
Chemical Fractionation
Crotalid Venoms / chemistry,  enzymology*
Cytoplasm / drug effects
DNA Fragmentation
DNA, Protozoan / drug effects
Hydrogen Peroxide / pharmacology
L-Amino Acid Oxidase / isolation & purification,  metabolism,  pharmacology*
Mitochondrial Swelling / drug effects
Trypanosoma cruzi / drug effects*
Chemical
Reg. No./Substance:
0/Antiprotozoal Agents; 0/Crotalid Venoms; 0/DNA, Protozoan; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.6/Catalase; EC 1.4.3.2/L-Amino Acid Oxidase

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