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L-amino acid biosensor based on L-amino acid oxidase immobilized onto NiHCNFe/c-MWCNT/PPy/GC electrode.
MedLine Citation:
PMID:  23237796     Owner:  NLM     Status:  Publisher    
A method is described for construction of a highly sensitive amperometric L-amino acid biosensor based on covalent immobilization of goat kidney L- amino acid oxidase (LAAO) onto carboxylated multiwalled carbon nanotubes/nickel hexacyanoferrate/polypyrrole hybrid film electrodeposited on surface of glassy carbon electrode. The biosensor showed optimum response within 5 s at pH 7.5 and 35°C, when polarized at 0.15V vs Ag/AgCl. There was a linear relationship between biosensor response (μA) and L-phenylalanine concentration in the range, 0.5μM to 100mM. The sensitivity of the biosensor was 79.31 nAcm(-2)μM(-1) with a detection limit of 0.5μM (S/N =3). The enzyme electrode was used 160 times over a period of 140 days, when stored dry at 4°C. The biosensor was evaluated and employed for determination of L-amino acid level in fruits juices and alcoholic beverages. The biosensor showed excellent analytical characteristics along with short response time, good shelf life, and negligible interference of commonly present interferents. The film could be used for improvement of other biosensors also.
Suman Lata; C S Pundir
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-10
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  -     ISSN:  1879-0003     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Department of Biochemistry, M.D.University, Rohtak-124 001, India.
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