Document Detail


L-Proline transport by isolated renal tubules from newborn and adult rats.
MedLine Citation:
PMID:  6835714     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proline uptake and metabolism has been examined in newborn Sprague-Dawley rat kidney and compared to that in adult animals. [14C]-Proline uptake by renal tubule fragments from newborn rats occurs at the same initial rate as in adult tubules, but at physiologic concentrations achieves significantly higher intracellular levels after 15 min of incubation. Considerable metabolism of the proline taken up was observed in tissue of both ages. Analysis of acid soluble and insoluble tubule fractions from newborn and adults indicates similar degrees of proline incorporation into protein and oxidation to CO2 relative to the amount of radioactivity taken up. A major difference exists, however, with respect to the labeled components of the acid extract: adult tubules convert [14C]-proline to metabolites at a rate twice that of newborn. Analysis of concentration-dependent uptake data reveals two distinct entry systems for proline in both isolated newborn and adult tubules. No difference in the Km or Vmax was found between the young and mature tubules.
Authors:
S M Hwang; M A Serabian; K S Roth; S Segal
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Pediatric research     Volume:  17     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  42-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Animals, Newborn
Female
Kidney Cortex / metabolism
Kidney Tubules / analysis,  metabolism*
Male
Proline / blood,  metabolism*,  urine
Rats
Rats, Inbred Strains
Time Factors
Grant Support
ID/Acronym/Agency:
AM 10894/AM/NIADDK NIH HHS; HD 08536/HD/NICHD NIH HHS; K04 HD00257/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
147-85-3/Proline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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