Document Detail


L-Leucine induces growth arrest and persistent ERK activation in glioma cells.
MedLine Citation:
PMID:  22009140     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glioma is the most common type of brain tumor, and has the worst prognosis in human malignancy. Experimental evidence suggests that the use of high concentrations of various amino acids may perturb neoplastic cell growth. Thus, the aim of this study was to investigate whether essential amino acids can alter the growth and proliferation of glioma cells. Studies were performed using C6 rat glioma cell lines. High concentration of L-leucine induced growth arrest of glioma cell lines. Terminal transferase uridyl nick end labeling assay and cell cycle analysis showed that the effect of L-leucine on glioma cells growth was not cytotoxic, but rather cytostatic. Additionally, the extracellular signal-regulated protein kinase was activated in L-leucine-treated glioma cells, and inhibition of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase 1 (MEK) enhanced the effect of L-leucine on glioma cell growth. These data suggest that high concentration L-leucine combined with inhibition of MEK is a potential strategy for glioma cell growth arrest.
Authors:
Satoru Takeuchi; Hiroshi Nawashiro; Kojiro Wada; Namiko Nomura; Terushige Toyooka; Naoki Otani; Hideo Osada; Hirotaka Matsuo; Nariyoshi Shinomiya
Publication Detail:
Type:  Journal Article     Date:  2011-10-19
Journal Detail:
Title:  Amino acids     Volume:  43     ISSN:  1438-2199     ISO Abbreviation:  Amino Acids     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-16     Completed Date:  2012-11-06     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  9200312     Medline TA:  Amino Acids     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  717-24     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan. drne776@ndmc.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Amino Acids, Cyclic / pharmacology
Animals
Antineoplastic Agents / pharmacology*
Apoptosis
Cell Cycle Checkpoints
Cell Line, Tumor
Cell Proliferation / drug effects*
Cell Shape / drug effects
Cell Survival / drug effects
Cyclin D1 / metabolism
Drug Synergism
Enzyme Activation
Extracellular Signal-Regulated MAP Kinases / metabolism*
Flavonoids / pharmacology
Glioma
JNK Mitogen-Activated Protein Kinases / metabolism
Large Neutral Amino Acid-Transporter 1 / metabolism
Leucine / pharmacology*
MAP Kinase Signaling System
Phosphorylation
Protein Processing, Post-Translational
Rats
p38 Mitogen-Activated Protein Kinases / metabolism
Chemical
Reg. No./Substance:
0/2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; 0/Amino Acids, Cyclic; 0/Antineoplastic Agents; 0/Ccnd1 protein, rat; 0/Flavonoids; 0/Large Neutral Amino Acid-Transporter 1; 136601-57-5/Cyclin D1; 20448-79-7/2-aminobicyclo(2,2,1)heptane-2-carboxylic acid; 61-90-5/Leucine; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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