| L-Carnitine alters nitric oxide synthase activity in fibroblasts depending on the peroxisomal status. | |
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MedLine Citation:
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PMID: 12479865 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fibroblast cellular models are widely used for research on fatty acid metabolism. Due to the importance of L-carnitine in intermediary metabolism we studied the effects of L-carnitine on healthy human skin fibroblasts and fibroblasts without functional peroxisomes (Zellweger Syndrome) cultivated under carnitine deficiency, which is caused by standard media compositions. The application of physiological (0.1mM) or super-physiological (1mM) doses of L-carnitine causes a significant decrease of the specific activity of nitric oxide synthase (NOS, 2.25+/-0.10 to 1.36 pmol/(minmg)+/-0.09 pmol/(minmg) at 0.1mM), proliferation and a tendentious decrease of the antioxidant defence potential against hydrogen peroxide only in control cells. Simultaneous application of L-carnitine and 100 micro M N-acetylcysteine (NAC) prevents the alterations in control cells. Thus, L-carnitine alters the cellular regulation of the NOS probably by reactive oxygen species (ROS), which suggests that carnitine deficient media neither reflect physiological conditions for cellular models for fatty acid metabolism nor for the regulation of NOS. |
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Authors:
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Thomas Koeck; Karl Kremser |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The international journal of biochemistry & cell biology Volume: 35 ISSN: 1357-2725 ISO Abbreviation: Int. J. Biochem. Cell Biol. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2002-12-13 Completed Date: 2003-09-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9508482 Medline TA: Int J Biochem Cell Biol Country: England |
Other Details:
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Languages: eng Pagination: 149-56 Citation Subset: IM |
Affiliation:
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Department of Medical Chemistry, School of Medicine, University of Vienna, Waehringerstrasse 10, Vienna A-1090, Austria. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology Animals Antioxidants / metabolism, pharmacology Carnitine / deficiency, pharmacology* Cattle Cells, Cultured Fatty Acids / analysis, blood Fibroblasts / drug effects, metabolism* Humans Hydrogen Peroxide / pharmacology Nitric Oxide Synthase / drug effects, metabolism* Nitric Oxide Synthase Type III Oxidative Stress Peroxisomes / drug effects, metabolism* Proteins / drug effects, metabolism Reference Values Toxicity Tests Zellweger Syndrome / pathology |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Fatty Acids; 0/Proteins; 541-15-1/Carnitine; 616-91-1/Acetylcysteine; 7722-84-1/Hydrogen Peroxide; EC 1.14.13.39/NOS3 protein, human; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type III |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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