Document Detail


Kynurenic acid enhances expression of p21 Waf1/Cip1 in colon cancer HT-29 cells.
MedLine Citation:
PMID:  22814028     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background: Kynurenic acid (KYNA), a tryptophan metabolite, was found in the mucus of rat small intestine. However, its role in the gastrointestinal tract is still not fully elucidated. Methods: To verify whether KYNA affects cell cycle regulators, the protein expression of cyclin-dependent kinase inhibitor p21 Waf1/Cip1 was investigated in colon adenocarcinoma HT-29 cells exposed to KYNA. MTT, BrdU assay and siRNA technology were used to evaluate the effect of KYNA on cancer cell proliferation. Results: KYNA significantly enhanced the expression of p21 Waf1/Cip1. Importantly, the overexpression of this protein was involved in inhibition of proliferation and DNA synthesis in HT-29 cells. Conclusions: KYNA may be considered as a potential chemoprevention agent against colon cancer.
Authors:
Katarzyna Walczak; Waldemar A Turski; Wojciech Rzeski
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pharmacological reports : PR     Volume:  64     ISSN:  1734-1140     ISO Abbreviation:  Pharmacol Rep     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-07-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234999     Medline TA:  Pharmacol Rep     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  745-50     Citation Subset:  IM    
Affiliation:
Department of Medical Biology, Institute of Rural Health, Jaczewskiego 2, PL 20-950 Lublin, Poland. walczak.ka@gmail.com.
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