| The Kv2.1 channels mediate neuronal apoptosis induced by excitotoxicity. | |
| | |
MedLine Citation:
|
PMID: 19077057 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Chronic loss of intracellular K(+) can induce neuronal apoptosis in pathological conditions. However, the mechanism by which the K(+) channels are regulated in this process remains largely unknown. Here, we report that the increased membrane expression of Kv2.1 proteins in cortical neurons deprived of serum, a condition known to induce K(+) loss, promotes neuronal apoptosis. The increase in I(K) current density and apoptosis in the neurons deprived of serum were inhibited by a dominant negative form of Kv2.1 and MK801, an antagonist to NMDA receptors. The membrane level of Kv2.1 and its interaction with SNAP25 were increased, whereas the Kv2.1 phosphorylation was inhibited in the neurons deprived of serum. Botulinum neurotoxin, an agent known to prevent formation of soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex, suppressed the increase in I(K) current density. Together, these results suggest that NMDA receptor-dependent Kv2.1 membrane translocation is regulated by a soluble N-ethylmaleimide-sensitive factor attachment protein receptor-dependent vesicular trafficking mechanism and is responsible for neuronal cell death induced by chronic loss of K(+). |
| | |
Authors:
|
Hailan Yao; Kechun Zhou; Dong Yan; Mingtao Li; Yizheng Wang |
Related Documents
:
|
16719817 - Survival signaling pathways activated by nmda receptors. 16504407 - Ecstasy-induced cell death in cortical neuronal cultures is serotonin 2a-receptor-depen... 11069947 - Neuronal apoptosis by apolipoprotein e4 through low-density lipoprotein receptor-relate... 11007787 - Coregulation of neurite outgrowth and cell survival by amphoterin and s100 proteins thr... 18426817 - Somatostatinergic ligands in dopamine-sensitive and -resistant prolactinomas. 9620997 - Genetically divergent strains of human immunodeficiency virus type 2 use multiple corec... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-10 |
Journal Detail:
|
Title: Journal of neurochemistry Volume: 108 ISSN: 1471-4159 ISO Abbreviation: J. Neurochem. Publication Date: 2009 Feb |
Date Detail:
|
Created Date: 2009-02-06 Completed Date: 2009-04-20 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
|
Languages: eng Pagination: 909-19 Citation Subset: IM |
Affiliation:
|
The Graduate School, Chinese Academy of Science, Shanghai, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / drug effects, physiology* Botulinum Toxins / pharmacology Cell Membrane / genetics, metabolism Cells, Cultured Cerebral Cortex / cytology, drug effects, metabolism* Culture Media, Serum-Free / pharmacology Excitatory Amino Acid Antagonists / pharmacology N-Ethylmaleimide-Sensitive Proteins / metabolism Neuromuscular Agents / pharmacology Neurons / drug effects, metabolism* Neurotoxins / metabolism*, pharmacology Patch-Clamp Techniques Potassium / metabolism Potassium Deficiency / metabolism*, physiopathology Protein Transport / drug effects, physiology Rats Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors, metabolism Shab Potassium Channels / drug effects, genetics, metabolism* Synaptosomal-Associated Protein 25 / metabolism Transport Vesicles / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Botulinum Toxins; 0/Culture Media, Serum-Free; 0/Excitatory Amino Acid Antagonists; 0/Kcnb1 protein, rat; 0/Neuromuscular Agents; 0/Neurotoxins; 0/Nsf protein, rat; 0/Receptors, N-Methyl-D-Aspartate; 0/Shab Potassium Channels; 0/Snap25 protein, rat; 0/Synaptosomal-Associated Protein 25; 7440-09-7/Potassium; EC 3.6.4.6/N-Ethylmaleimide-Sensitive Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Repeated alcohol administration during adolescence causes changes in the mesolimbic dopaminergic and...
Next Document: Isolation and characterization of new exon 11-associated N-terminal splice variants of the human mu ...