Document Detail

Kv1.3 potassium channels in human alveolar macrophages.
MedLine Citation:
PMID:  12909584     Owner:  NLM     Status:  MEDLINE    
Human alveolar macrophages were obtained from macroscopically normal lung tissue obtained at surgical resections, isolated by adherence, and identified by morphology. Whole cell recordings were made from cells 1-3 h in culture, using electrodes containing potassium chloride. From a holding potential of -100 mV, depolarizing pulses to -40 mV or greater activated an outward current. Tail current reversals showed that this current was potassium selective. Margatoxin completely blocked the current; the concentration giving half-maximal block was 160 pM. In current clamp recordings, the resting membrane potential was -34 mV; margatoxin depolarized cells to close to 0 mV. A pure macrophage population was isolated by fluorescence-activated cell sorting, using the phagocytosis of BODIPY-labeled zymosan particles. Reverse transcription-polymerase chain reaction showed that, of 13 voltage-gated K+ (Kv) potassium channels sought, only Kv1.3 mRNA was present. Margatoxin (1 nM) did not affect the percentage of cells showing phagocytosis sorted from the total population. Under these experimental conditions Kv1.3 sets the resting potential of the cells, but it is not required for Fc receptor-mediated phagocytosis.
Amanda B Mackenzie; Hari Chirakkal; R Alan North
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-08-08
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  285     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-08     Completed Date:  2003-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L862-8     Citation Subset:  IM    
Inst. of Molecular Physiology, Alfred Denny Bldg., Western Bank, Univ. of Sheffield, Sheffield S10 2TN, United Kingdom.
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MeSH Terms
Interleukin-1 / metabolism
Kv1.3 Potassium Channel
Macrophages, Alveolar / drug effects,  metabolism*,  physiology
Neurotoxins / pharmacology
Osmolar Concentration
Patch-Clamp Techniques
Phagocytosis / drug effects
Potassium Channels / genetics,  metabolism*
Potassium Channels, Voltage-Gated*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Scorpion Venoms
Reg. No./Substance:
0/Interleukin-1; 0/KCNA3 protein, human; 0/Kv1.3 Potassium Channel; 0/Neurotoxins; 0/Potassium Channels; 0/Potassium Channels, Voltage-Gated; 0/RNA, Messenger; 0/Scorpion Venoms; 145808-47-5/margatoxin

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