Document Detail


Kojic acid production by Aspergillus flavus using gelatinized and hydrolyzed sago starch as carbon sources.
MedLine Citation:
PMID:  9867479     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Direct conversion of gelatinized sago starch into kojic acid by Aspergillus flavus strain having amylolytic enzymes was carried out at two different scales of submerged batch fermentation in a 250-mL shake flask and in a 50-L stirred-tank fermentor. For comparison, fermentations were also carried out using glucose and glucose hydrolyzate from enzymic hydrolysis of sago starch as carbon sources. During kojic acid fermentation of starch, starch was first hydrolyzed to glucose by the action of alpha-amylase and glucoamylase during active growth phase. The glucose remaining during the production phase (non-growing phase) was then converted to kojic acid. Kojic acid production (23.5 g/L) using 100 g/L sago starch in a shake flask was comparable to fermentation of glucose (31.5 g/L) and glucose hydrolyzate (27.9 g/L) but in the 50-L fermentor was greatly reduced due to non-optimal aeration conditions. Kojic acid production using glucose was higher in the 50-L fermentor than in the shake flask.
Authors:
M Rosfarizan; A B Ariff; M A Hassan; M I Karim
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Folia microbiologica     Volume:  43     ISSN:  0015-5632     ISO Abbreviation:  Folia Microbiol. (Praha)     Publication Date:  1998  
Date Detail:
Created Date:  1998-12-11     Completed Date:  1998-12-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376757     Medline TA:  Folia Microbiol (Praha)     Country:  CZECH REPUBLIC    
Other Details:
Languages:  eng     Pagination:  459-64     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Universiti Putra Malaysia, Selangor.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus flavus / growth & development,  metabolism*
Fermentation
Glucan 1,4-alpha-Glucosidase / metabolism
Glucose / metabolism
Hydrolysis
Mycotoxins / biosynthesis*
Pyrones / metabolism*
Starch / metabolism*
alpha-Amylases / metabolism
Chemical
Reg. No./Substance:
0/Mycotoxins; 0/Pyrones; 50-99-7/Glucose; 501-30-4/kojic acid; 9005-25-8/Starch; EC 3.2.1.1/alpha-Amylases; EC 3.2.1.3/Glucan 1,4-alpha-Glucosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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