| Knockdown of glycogen synthase kinase 3 beta attenuates 6-hydroxydopamine-induced apoptosis in SH-SY5Y cells. | |
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MedLine Citation:
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PMID: 20920551 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glycogen synthase kinase 3 beta (GSK3β) plays a critical role in signal transductions concerning neuronal death. In the present study, we investigated the potential role of GSK3β in 6-hydroxydopamine (6-OHDA)-induced toxicity in human neuroblastoma cell line SH-SY5Y. We assessed the apoptotic proteins and the relative levels of pGSK3β (Ser9) and pGSK3β (Tyr216) to GSK3β in 6-OHDA-treated SH-SY5Y. Furthermore, we downregulated the expression of GSK3β by short hairpin RNA (shRNA) interference and compared the cell viability and expression of apoptotic proteins in knockdown group with those in control group under the treatment of 6-OHDA. We found that 6-OHDA increased the expression of caspase-3 and caspase-9 but not caspase-8. Additionally, 6-OHDA decreased the ratio of pGSK3β (Ser9)/GSK3β and increased the ratio of pGSK3β (Tyr216)/GSK3β. Moreover, 6-OHDA induced less cell viability loss and lower expression of caspase-9 and caspase-3 in GSK3β knockdown group compared with control. The present data indicate that 6-OHDA may induce apoptosis in SH-SY5Y via the intrinsic death pathway and GSK3β knockdown can partly attenuate 6-OHDA-induced neuronal death and apoptosis, suggesting that GSK3β may have the potential to serve as a therapeutic target for PD. |
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Authors:
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Yi Li; Feifei Luo; Lei Wei; Zhuolin Liu; Pingyi Xu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-22 |
Journal Detail:
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Title: Neuroscience letters Volume: 487 ISSN: 1872-7972 ISO Abbreviation: Neurosci. Lett. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2011-03-09 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 41-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenergic Agents
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toxicity* Analysis of Variance Apoptosis / drug effects* Caspases / metabolism Cell Count / methods Cell Line, Tumor Glycogen Synthase Kinase 3 / deficiency*, genetics Humans Neuroblastoma / pathology Oxidopamine / toxicity* RNA, Small Interfering / pharmacology Tetrazolium Salts / diagnostic use Thiazoles / diagnostic use Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Adrenergic Agents; 0/RNA, Small Interfering; 0/Tetrazolium Salts; 0/Thiazoles; 1199-18-4/Oxidopamine; 298-93-1/thiazolyl blue; EC 2.7.11.1/glycogen synthase kinase 3 beta; EC 2.7.11.26/Glycogen Synthase Kinase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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