Document Detail


Knockdown of eIF4E suppresses cell growth and migration, enhances chemosensitivity and correlates with increase in Bax/Bcl-2 ratio in triple-negative breast cancer cells.
MedLine Citation:
PMID:  20652449     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Elevated activity of the eukaryotic translation initiation factor 4E (eIF4E) plays crucial roles in tumorigenesis and disease progression by disproportionately increasing translation of mRNAs coding proteins that play significant roles in all aspects of malignancy, providing that eIF4E as an attractive target for therapeutic intervention. In this study, we showed that inhibition of eIF4E by small interfering RNAs (siRNA) resulted in cell cycle arrest and suppression of colony formation in MDA-MB-231 triple-negative (TN) breast cancer cells. Migration transwell assay revealed that repression of eIF4E effectively inhibited motility of MDA-MB-231 cancer cells. Importantly, we showed that silencing of eIF4E sensitized MDA-MB-231 cells to chemotherapeutic drugs of cisplatin, adriamycin, paclitaxel and docetaxel as assessed by MTT assay. Moreover, Western blot assay showed that eIF4E siRNA increased Bax/Bcl-2 ratio in MDA-MB-231 cells. Taken together, we showed that knockdown of eIF4E suppressed cell growth and migration, enhanced chemosensitivity, suggesting a potential therapeutic target in TN breast carcinoma.
Authors:
Fei-Fei Zhou; Min Yan; Gui-Fang Guo; Fang Wang; Hui-Juan Qiu; Fei-Meng Zheng; Yan Zhang; Qiang Liu; Xiao-Feng Zhu; Liang-Ping Xia
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Publication Detail:
Type:  Journal Article     Date:  2010-07-22
Journal Detail:
Title:  Medical oncology (Northwood, London, England)     Volume:  28     ISSN:  1559-131X     ISO Abbreviation:  Med. Oncol.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9435512     Medline TA:  Med Oncol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1302-7     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, 651 Dongfeng Road East, 510060, Guangzhou, China.
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