Document Detail

Knockdown of V-ATPase subunit A (atp6v1a) impairs acid secretion and ion balance in zebrafish (Danio rerio).
MedLine Citation:
PMID:  17272665     Owner:  NLM     Status:  MEDLINE    
In the skin of zebrafish embryo, the vacuolar H(+)-ATPase (V-ATPase, H(+) pump) distributed mainly in the apical membrane of H(+)-pump-rich cells, which pump internal acid out of the embryo and function similarly to acid-secreting intercalated cells in mammalian kidney. In addition to acid excretion, the electrogenic H(+) efflux via the H(+)-ATPases in the gill apical membrane of freshwater fish was proposed to act as a driving force for Na(+) entry through the apical Na(+) channels. However, convincing molecular physiological evidence in vivo for this model is still lacking. In this study, we used morpholino-modified antisense oligonucleotides to knockdown the gene product of H(+)-ATPase subunit A (atp6v1a) and examined the phenotype of the mutants. The H(+)-ATPase knockdown embryos revealed several abnormalities, including suppression of acid-secretion from skin, growth retardation, trunk deformation, and loss of internal Ca(2+) and Na(+). This finding reveals the critical role of H(+)-ATPase in embryonic acid -secretion and ion balance, as well.
Jiun-Lin Horng; Li-Yih Lin; Chang-Jen Huang; Fumi Katoh; Toyoji Kaneko; Pung-Pung Hwang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-01
Journal Detail:
Title:  American journal of physiology. Regulatory, integrative and comparative physiology     Volume:  292     ISSN:  0363-6119     ISO Abbreviation:  Am. J. Physiol. Regul. Integr. Comp. Physiol.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-03     Completed Date:  2007-07-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901230     Medline TA:  Am J Physiol Regul Integr Comp Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  R2068-76     Citation Subset:  IM    
Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan, ROC.
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MeSH Terms
Acids / metabolism*
Embryo, Nonmammalian / metabolism*
Fresh Water
Gene Expression Regulation, Enzymologic
Protein Subunits / deficiency,  genetics,  metabolism
Proton-Translocating ATPases / chemistry,  deficiency*,  genetics,  metabolism
Vacuolar Proton-Translocating ATPases / deficiency,  genetics*,  metabolism*
Water-Electrolyte Balance*
Yolk Sac / metabolism
Zebrafish / metabolism*
Zebrafish Proteins / deficiency,  genetics*,  metabolism*
Reg. No./Substance:
0/Acids; 0/Protein Subunits; 0/Protons; 0/Zebrafish Proteins; EC 3.6.1.-/V-ATPase subunit A, zebrafish; EC 3.6.1.-/Vacuolar Proton-Translocating ATPases; EC ATPases

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