Document Detail

Knockdown of NPM1 by RNA interference inhibits cells proliferation and induces apoptosis in leukemic cell line.
MedLine Citation:
PMID:  21537492     Owner:  NLM     Status:  MEDLINE    
Nucleophosmin (NPM1) is an abundant and ubiquitously expressed phosphoprotein that is known to influence solid tumors progression. However, little is known about the role of NPM1 in leukemia. Here, we knocked down the NPM1 expression by RNA interference to investigate the role of NPM1 in leukemic cells proliferation and apoptosis. The interference vector pNPM1-shRNA was constructed and transfected into the human leukemic K562 cell line. The expression levels of NPM1 mRNA and protein were detected by quantitative real-time PCR and Western blot, respectively. Cells proliferation potential in vitro was assessed by methyl thiazolyl tetrazolium (MTT) and colony formation assays. Flow cytometry was used to detect the distribution of cell cycle. Cellular apoptosis was reflected by the relative activities of caspase-3 and caspase-8. The results showed that the expression levels of NPM1 mRNA and protein in K562 cells were significantly reduced after pNPM1-shRNA transfection. The cells growth was significantly inhibited in a time-dependent manner and the number of colonies was significantly reduced in the pNPM1-shRNA transfected cells. Meanwhile, the percentage of cells in G1 phase in the K562/pNPM1-shRNA cells was significantly increased. In addition, there were higher relative activities of caspase-3/8 in the pNPM1-shRNA transfected cells. These results indicate that down-regulation of NPM1 expression inhibits leukemic cells proliferation, blocks cell cycle progression and induces cellular apoptosis. It may implicate a potential target for leukemia gene therapy.
Feng-Xian Qin; Hui-Yuan Shao; Xian-Chun Chen; Shi Tan; Hui-Juan Zhang; Zong-Yu Miao; Li Wang; Hui-Chen; Ling Zhang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-20
Journal Detail:
Title:  International journal of medical sciences     Volume:  8     ISSN:  1449-1907     ISO Abbreviation:  Int J Med Sci     Publication Date:  2011  
Date Detail:
Created Date:  2011-05-03     Completed Date:  2011-08-30     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101213954     Medline TA:  Int J Med Sci     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  287-94     Citation Subset:  IM    
Copyright Information:
© Ivyspring International Publisher.
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
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MeSH Terms
Apoptosis / genetics,  physiology*
Cell Line, Tumor
Cell Proliferation
Flow Cytometry
Leukemia / genetics,  metabolism*
Nuclear Proteins / genetics*,  metabolism*
RNA Interference / physiology
Reg. No./Substance:
0/Nuclear Proteins; 117896-08-9/nucleophosmin

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