| Knockdown of MBP-1 in human prostate cancer cells delays cell cycle progression. | |
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MedLine Citation:
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PMID: 16762917 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that MBP-1 acts as a general transcriptional repressor, and forced expression of MBP-1 exerts an anti-proliferative effect on a number of human cancer cells. In this report, we have investigated the role of endogenous MBP-1 in cell growth regulation. For this, we generated human prostate cancer cells (PC3) stably transfected with short hairpin RNA targeting MBP-1. We have observed retarded growth and longer doubling time of MBP-1 knockdown PC3 cells as compared with control mock-transfected PC3 cells. Fluorescence-activated cell sorter analysis suggested that PC3 cells expressing MBP-1-specific small interfering RNA accumulated during G2/M phase of the cell cycle. Further analysis suggested that depletion of MBP-1 was associated with reduction of cyclin A and cyclin B1 expression when compared with that of the control cells. A delayed induction of cyclin A and B1 expression was observed in MBP-1-depleted PC3 cells (PC3-4.2) upon serum stimulation, although the level of expression was much lower than that of control PC3 cells. Supplementation of MBP-1 in PC3-4.2 cells restored cyclin A and cyclin B1 expression. Together, these results suggest that knockdown of MBP-1 in prostate cancer cells perturbs cell proliferation by inhibiting cyclin A and cyclin B1 expression. |
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Authors:
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Asish K Ghosh; Robert Steele; Ratna B Ray |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-06-08 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 281 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2006-08-14 Completed Date: 2006-09-25 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 23652-7 Citation Subset: IM |
Affiliation:
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Department of Pathology, Saint Louis University, St. Louis, Missouri 63104, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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physiology* Cell Line Cell Line, Tumor Cell Proliferation Cell Size Cyclin A / antagonists & inhibitors, biosynthesis Cyclin B / antagonists & inhibitors, biosynthesis Cyclin B1 Growth Inhibitors / antagonists & inhibitors*, biosynthesis, genetics, physiology Humans Male Nerve Tissue Proteins / antagonists & inhibitors*, genetics*, physiology Prostatic Neoplasms / metabolism*, pathology*, prevention & control RNA Interference / physiology* Repressor Proteins / antagonists & inhibitors*, biosynthesis, genetics, physiology Time Factors Transcription Factors / antagonists & inhibitors*, genetics*, physiology Transfection |
| Grant Support | |
ID/Acronym/Agency:
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CA52799/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CCNB1 protein, human; 0/Cyclin A; 0/Cyclin B; 0/Cyclin B1; 0/Growth Inhibitors; 0/MBP protein, human; 0/Nerve Tissue Proteins; 0/Repressor Proteins; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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