| Knockdown of FoxM1 by siRNA interference decreases cell proliferation, induces cell cycle arrest and inhibits cell invasion in MHCC-97H cells in vitro. | |
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MedLine Citation:
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PMID: 20154714 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the effects of small interfering RNA (siRNA) knockdown of forkhead box M1 (FoxM1) on the proliferation and invasion capacities of human hepatocellular carcinoma MHCC-97H cells in vitro. METHODS: The expression levels of FoxM1 in human hepatocellular carcinoma samples, adjacent non-hepatocellular carcinoma liver samples and MHCC-97 cell lines were detected by RT-PCR and Western blotting. FoxM1 siRNA was transfected into MHCC-97H cells with Lipofectamine 2000. Cell growth was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell cycle analysis was performed by flow cytometry. Protein expression levels were evaluated by Western blotting. Anchorage-independent growth and the invasive potency of MHCC-97H cells were measured by soft agar colony formation and a transwell cell invasion assay, respectively. RESULTS: FoxM1 was over-expressed in hepatocellular carcinoma samples compared to adjacent non-hepatocellular carcinoma liver samples. FoxM1 siRNA was successfully transfected into MHCC-97H cells, resulting in the significant inhibition of FoxM1 mRNA and protein expression. Down-regulation of FoxM1 inhibited cell proliferation, caused cell cycle arrest, and decreased invasion of MHCC-97H cells. Compared with control and mock groups, the FoxM1 siRNA transfected cells showed decreased protein expressions of cyclin B1 and cyclin D1, whereas p27 protein expression was increased. Down-regulation of FoxM1 reduced the expression of matrix metalloproteinase-2 (MMP-2) and urokinase plasminogen activator (uPA). CONCLUSION: FoxM1 is functionally involved in hepatocellular carcinoma cell proliferation and invasion and is a potential target for hepatocellular carcinoma therapy. |
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Authors:
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Qi-fei Wu; Chang Liu; Ming-hui Tai; Dong Liu; Lei Lei; Rui-tao Wang; Min Tian; Yi L? |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-15 |
Journal Detail:
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Title: Acta pharmacologica Sinica Volume: 31 ISSN: 1745-7254 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-03 Completed Date: 2010-06-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: United States |
Other Details:
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Languages: eng Pagination: 361-6 Citation Subset: IM |
Affiliation:
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Department of Hepatobiliary Surgery, the First Affiliated Hospital, Xi'an Jiaotong University, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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genetics,
metabolism*,
pathology Cell Cycle* Cell Proliferation* Forkhead Transcription Factors / genetics*, metabolism Gene Expression Regulation, Neoplastic Gene Silencing Humans Liver Neoplasms / genetics, metabolism*, pathology Neoplasm Invasiveness / genetics RNA, Small Interfering / genetics* |
| Chemical | |
Reg. No./Substance:
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0/FOXM1 protein, human; 0/Forkhead Transcription Factors; 0/RNA, Small Interfering |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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