| Knockdown of F1 epsilon subunit decreases mitochondrial content of ATP synthase and leads to accumulation of subunit c. | |
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MedLine Citation:
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PMID: 20026007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The subunit epsilon of mitochondrial ATP synthase is the only F1 subunit without a homolog in bacteria and chloroplasts and represents the least characterized F1 subunit of the mammalian enzyme. Silencing of the ATP5E gene in HEK293 cells resulted in downregulation of the activity and content of the mitochondrial ATP synthase complex and of ADP-stimulated respiration to approximately 40% of the control. The decreased content of the epsilon subunit was paralleled by a decrease in the F1 subunits alpha and beta and in the Fo subunits a and d while the content of the subunit c was not affected. The subunit c was present in the full-size ATP synthase complex and in subcomplexes of 200-400 kDa that neither contained the F1 subunits, nor the Fo subunits. The results indicate that the epsilon subunit is essential for the assembly of F1 and plays an important role in the incorporation of the hydrophobic subunit c into the F1-c oligomer rotor of the mitochondrial ATP synthase complex. |
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Authors:
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Vendula Havlícková; Vilma Kaplanová; Hana Nůsková; Zdenek Drahota; Josef Houstek |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-21 |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1797 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2010 Jun-Jul |
Date Detail:
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Created Date: 2010-06-21 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1124-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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Department of Bioenergetics, Institute of Physiology and Centre for Applied Genomics, Academy of Sciences of the Czech Republic, 142 20 Prague. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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biosynthesis Base Sequence Gene Knockdown Techniques HEK293 Cells Humans Mitochondria / enzymology* Mitochondrial Proton-Translocating ATPases / antagonists & inhibitors*, chemistry, genetics, metabolism* Oxidative Phosphorylation Protein Subunits Proteins / antagonists & inhibitors*, chemistry, genetics, metabolism* RNA Interference RNA, Small Interfering / genetics |
| Chemical | |
Reg. No./Substance:
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0/ATPase inhibitory protein; 0/Protein Subunits; 0/Proteins; 0/RNA, Small Interfering; 56-65-5/Adenosine Triphosphate; EC 3.6.3.-/Mitochondrial Proton-Translocating ATPases |
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