Document Detail


Knockdown of Cyclophilin D Gene by RNAi Protects Rat from Ischemia/ Reperfusion-Induced Renal Injury.
MedLine Citation:
PMID:  20588055     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background/Aims: Mitochondrial permeability transition has a critical role in ischemia/reperfusion (I/R)-induced kidney injury. It is thought that mitochondrial permeability transition occurs after the opening of the permeability transition pore, a channel which putatively consists of a voltage-dependent anion channel, adenine nucleotide translocator and cyclophilin D (CypD). Much evidence shows that CypD plays an important role in I/R-induced injury. Methods: To evaluate the role of CypD following I/R renal injury, we tested the hypothesis that knockdown of CypD gene by RNA interference (RNAi) protects rat from I/R-induced renal injury. Results: Our data show that knockdown of CypD by RNAi protects normal rat kidney cell line from hypoxia-induced necrotic death. Infection of lentivirus expressing CypD RNAi sequence produces a significant reduction of CypD at both mRNA and protein levels. Both pathologic and biochemical analyses show that knockdown of CypD by RNAi protects rat kidney from I/R-induced renal injury. Conclusion: Our study provides the evidence that CypD may be a potential target for protecting I/R-induced renal injury.
Authors:
Wei Hu; Zhiqiang Chen; Zhangqun Ye; Ding Xia; Zhongyu Xia; Jiefeng Ma; Min Zhu; Gang Chen
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-6-24
Journal Detail:
Title:  Kidney & blood pressure research     Volume:  33     ISSN:  1423-0143     ISO Abbreviation:  -     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-7-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610505     Medline TA:  Kidney Blood Press Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  193-199     Citation Subset:  -    
Copyright Information:
Copyright © 2010 S. Karger AG, Basel.
Affiliation:
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
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