| Knockdown of Cyclophilin D Gene by RNAi Protects Rat from Ischemia/ Reperfusion-Induced Renal Injury. | |
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MedLine Citation:
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PMID: 20588055 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background/Aims: Mitochondrial permeability transition has a critical role in ischemia/reperfusion (I/R)-induced kidney injury. It is thought that mitochondrial permeability transition occurs after the opening of the permeability transition pore, a channel which putatively consists of a voltage-dependent anion channel, adenine nucleotide translocator and cyclophilin D (CypD). Much evidence shows that CypD plays an important role in I/R-induced injury. Methods: To evaluate the role of CypD following I/R renal injury, we tested the hypothesis that knockdown of CypD gene by RNA interference (RNAi) protects rat from I/R-induced renal injury. Results: Our data show that knockdown of CypD by RNAi protects normal rat kidney cell line from hypoxia-induced necrotic death. Infection of lentivirus expressing CypD RNAi sequence produces a significant reduction of CypD at both mRNA and protein levels. Both pathologic and biochemical analyses show that knockdown of CypD by RNAi protects rat kidney from I/R-induced renal injury. Conclusion: Our study provides the evidence that CypD may be a potential target for protecting I/R-induced renal injury. |
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Authors:
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Wei Hu; Zhiqiang Chen; Zhangqun Ye; Ding Xia; Zhongyu Xia; Jiefeng Ma; Min Zhu; Gang Chen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-6-24 |
Journal Detail:
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Title: Kidney & blood pressure research Volume: 33 ISSN: 1423-0143 ISO Abbreviation: - Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-7-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9610505 Medline TA: Kidney Blood Press Res Country: - |
Other Details:
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Languages: ENG Pagination: 193-199 Citation Subset: - |
Copyright Information:
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Copyright © 2010 S. Karger AG, Basel. |
Affiliation:
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Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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