Document Detail

Knockdown of CD146 reduces the migration and proliferation of human endothelial cells.
MedLine Citation:
PMID:  16541130     Owner:  NLM     Status:  MEDLINE    
Our previous study has demonstrated that CD146 molecule is a biomarker on vascular endothelium, which is involved in angiogenesis and tumor growth. However the mechanism behind is not clear. Here we have for the first time developed a novel CD146 blockade system using CD146 siRNA to study its function on endothelial cells. Our data showed that CD146 siRNA specifically blocked the expression of CD146 on both mRNA and protein levels, leading to the significant suppression of HUVEC proliferation, adhesion and migration. These results demonstrate that CD146 plays a key role in vascular endothelial cell activity and angiogenesis, and CD146 siRNA can be used as a new inhibitor for anti-angiogenesis therapy.
Yanyong Kang; Fengcai Wang; Jing Feng; Dongling Yang; Xu Yang; Xiyun Yan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell research     Volume:  16     ISSN:  1001-0602     ISO Abbreviation:  Cell Res.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-16     Completed Date:  2006-06-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9425763     Medline TA:  Cell Res     Country:  China    
Other Details:
Languages:  eng     Pagination:  313-8     Citation Subset:  IM    
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China.
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MeSH Terms
Antigens, CD146 / drug effects*,  genetics
Cell Adhesion / drug effects
Cell Movement / drug effects*
Cell Proliferation / drug effects*
Cells, Cultured
Endothelial Cells / cytology*,  physiology
Endothelium, Vascular / cytology
Gene Expression Regulation
Neovascularization, Physiologic
RNA, Messenger / drug effects
RNA, Small Interfering / pharmacology*
Wound Healing / physiology
Reg. No./Substance:
0/Antigens, CD146; 0/RNA, Messenger; 0/RNA, Small Interfering

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