| Knock-down of postsynaptic density protein 95 expression by antisense oligonucleotides protects against apoptosis-like cell death induced by oxygen-glucose deprivation in vitro. | |
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MedLine Citation:
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PMID: 22233891 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Objective Postsynaptic density protein 95 (PSD-95) plays important roles in the regulation of glutamate signaling, such as that of N-methyl-D-aspartate receptors (NMDARs). In this study, the functional roles of PSD-95 in tyrosine phosphorylation of NMDAR subunit 2A (NR2A) and in apoptosis-like cell death induced by oxygen-glucose deprivation (OGD) in cultured rat cortical neurons were investigated. Methods We used immunoprecipitation and immunoblotting to detect PSD-95 protein level, tyrosine phosphorylation level of NR2A, and the interaction between PSD-95 and NR2A or Src. Apoptosis-like cells were observed by 4,6-diamidino-2-phenylindole staining. Results Tyrosine phosphorylation of NR2A and apoptosis-like cell death were increased after recovery following 60-min OGD. The increases were attenuated by pretreatment with antisense oligonucleotides against PSD-95 before OGD, but not by missense oligonucleotides or vehicle. PSD-95 antisense oligonucleotides also inhibited the increased interaction between PSD-95 and NR2A or Src, while NR2A expression did not change under this condition. Conclusion PSD-95 may be involved in regulating NR2A tyrosine phosphorylation by Src kinase. Inhibition of PSD-95 expression can be neuroprotective against apoptosis-like cell death after recovery from OGD. |
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Authors:
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Jing-Zhi Yan; Yong Liu; Yan-Yan Zong; Guang-Yi Zhang |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroscience bulletin Volume: 28 ISSN: 1995-8218 ISO Abbreviation: Neurosci Bull Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101256850 Medline TA: Neurosci Bull Country: China |
Other Details:
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Languages: eng Pagination: 69-76 Citation Subset: IM |
Affiliation:
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Jiangsu Key Laboratory of Brain Disease Bioinformation, Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002 China; E-mail: gyzhang@xzmc.edu.cn. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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