Document Detail


Knock-down of ERM family member moesin in host cells increases HIV-1 replication.
MedLine Citation:
PMID:  21486194     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Moesin is a member of the ERM (ezrin, radixin, moesin) family of cytoskeleton / membrane structure organizing and signal transduction proteins. Previously, we found an increased expression of moesin during HIV-1 infection. Moesin was also reported to be incorporated into HIV-1 virions. In order to analyze whether moesin is a host factor affecting the replication cycle of human immunodeficiency virus type 1 (HIV-1), we used small interfering RNAs (siRNAs) to evaluate the effect of moesin knockdown on HIV-1 replication in P4-CCR5 cells. Moesin´s knockdown did not affect the cell viability or cell phenotype. Interestingly, we observed a marked increase in viral replication, as demonstrated by enhanced HIV-1 RNA, p24 antigen and ß-galactosidase reporter expression. Moesin-dependent enhancement of HIV-1 replication was confirmed in lymphocytic host cells (Jurkat). These results suggest an overall rather restrictive role of moesin for HIV-1 replication in host cells in vitro.
Authors:
Gianni Capalbo; Thea Mueller-Kuller; Sandra Markovic; Stefan A Klein; Ursula Dietrich; Dieter Hoelzer; Oliver G Ottmann; Urban Johannes Scheuring
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-13
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  -     ISSN:  1931-8405     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
J. W. Goethe University Hospital, Department of Hematology / Oncology and Infectious Diseases, Frankfurt / Main, Germany; g.capalbo@blutspende.de.
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