Document Detail

Kluyveromyces as a host for heterologous gene expression: expression and secretion of prochymosin.
MedLine Citation:
PMID:  1366557     Owner:  NLM     Status:  MEDLINE    
We have developed the yeast Kluyveromyces lactis as a host organism for the production of the milk-clotting enzyme chymosin. In contrast to Saccharomyces cerevisiae, we found that this yeast is capable of the synthesis and secretion of fully active prochymosin. Various signal sequences could be used to efficiently direct the secretion of prochymosin in Kluyveromyces, but not in S. cerevisiae. We conclude that the efficient synthetic and secretory capacity of this heterologous protein is a property of the yeast Kluyveromyces. These results have led to the development of a large scale production process for chymosin.
J A van den Berg; K J van der Laken; A J van Ooyen; T C Renniers; K Rietveld; A Schaap; A J Brake; R J Bishop; K Schultz; D Moyer
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Bio/technology (Nature Publishing Company)     Volume:  8     ISSN:  0733-222X     ISO Abbreviation:  Biotechnology (N.Y.)     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-07-26     Completed Date:  1990-07-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8309273     Medline TA:  Biotechnology (N Y)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  135-9     Citation Subset:  B    
Royal Gist-brocades, Research and Development, Ma Delft, The Netherlands.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Chymosin / biosynthesis*,  genetics,  secretion
Cloning, Molecular
Enzyme Precursors / biosynthesis*,  genetics,  secretion
Kluyveromyces / genetics*,  metabolism
Molecular Sequence Data
Protein Processing, Post-Translational
Protein Sorting Signals / genetics,  physiology
Recombinant Fusion Proteins / biosynthesis*,  genetics,  secretion
Saccharomyces cerevisiae / genetics
Saccharomycetales / genetics*
Species Specificity
Reg. No./Substance:
0/Enzyme Precursors; 0/Protein Sorting Signals; 0/Recombinant Fusion Proteins; EC 3.4.23.-/prorennin; EC

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