Document Detail

Klebsiella pneumoniae secretes outer membrane vesicles that induce the innate immune response.
MedLine Citation:
PMID:  22428779     Owner:  NLM     Status:  Publisher    
Outer membrane vesicles (OMVs) that are derived from pathogenic Gram-negative bacteria are an important vehicle to deliver effector molecules to host cells, but the production of OMVs from Klebsiella pneumoniae, an important opportunistic pathogen of both nosocomial and community-acquired infections, and their role in bacterial pathogenesis have not yet been determined. In the present study, we examined the production of OMVs from K. pneumoniae and determined the induction of the innate immune response against K. pneumoniae OMVs. K. pneumoniae ATCC 13883 produced and secreted OMVs during in vitro culture. A proteomic analysis revealed that 159 different proteins were associated with K. pneumoniae OMVs. K. pneumoniae OMVs did not inhibit cell growth or induce cell death. However, these vesicles induced expression of pro-inflammatory cytokine genes such as interleukin (IL)-1β and IL-8 in epithelial cells. An intratracheal challenge of K. pneumoniae OMVs in neutropenic mice resulted in severe lung pathology similar to K. pneumoniae infection. In conclusion, K. pneumoniae produces OMVs like other pathogenic Gram-negative bacteria and K. pneumoniae OMVs are a molecular complex that induces the innate immune response. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Je Chul Lee; Eun Jeoung Lee; Jung Hwa Lee; So Hyun Jun; Chi Won Choi; Seung Il Kim; Sang Sun Kang; Sunghee Hyun
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-19
Journal Detail:
Title:  FEMS microbiology letters     Volume:  -     ISSN:  1574-6968     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Departement of Microbiology, Kyungpook National University School of Medicine, Daegu, Korea.
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