Document Detail

Kisspeptin cells in the ewe brain respond to leptin and communicate with neuropeptide Y and proopiomelanocortin cells.
MedLine Citation:
PMID:  20207832     Owner:  NLM     Status:  MEDLINE    
Kisspeptin stimulates reproduction, and kisspeptin cells in the arcuate nucleus (ARC) express Ob-Rb in the mouse. Herein we report studies in ewes to determine whether kisspeptin cells express Ob-Rb and respond to leptin and whether reciprocal connections exist between kisspeptin cells and proopiomelanocortin (POMC) or neuropeptide Y (NPY) cells to modulate reproduction and metabolic function. Kiss1 mRNA was measured by in situ hybridization in ovariectomized ewes that were normal body weight, lean, or lean with leptin treatment by intracerebroventricular (icv) infusion (4 microg/h, 3 d). Kiss1 expression in the ARC and the preoptic area was lower in hypogonadotropic lean animals than animals of normal weight, and icv infusion of leptin partially restored Kiss1 expression in lean animals. Single-cell laser capture microdissection coupled with real-time PCR showed that Kiss1 cells in the preoptic area and ARC express Ob-Rb. Double-label fluorescent immunohistochemistry showed that reciprocal connections exist between kisspeptin cells and NPY and POMC cells. Accordingly, we treated ovariectomized ewes with kisspeptin (5 microg/h, icv) or vehicle for 20 h and examined POMC and NPY gene expression by in situ hybridization. Kisspeptin treatment reduced POMC and increased NPY gene expression. Thus, kisspeptin neurons respond to leptin and expression of Kiss1 mRNA is affected by leptin status. Kisspeptin cells communicate with NPY and POMC cells, altering expression of the relevant genes in the target cells; reciprocal connections also exist. This network between the three cell types could coordinate brain control of reproduction and metabolic homeostatic systems.
Kathryn Backholer; Jeremy T Smith; Alix Rao; Alda Pereira; Javed Iqbal; Satoshi Ogawa; Qun Li; Iain J Clarke
Publication Detail:
Type:  Journal Article     Date:  2010-03-05
Journal Detail:
Title:  Endocrinology     Volume:  151     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-22     Completed Date:  2010-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2233-43     Citation Subset:  AIM; IM    
Department of Physiology, Monash University, Clayton, Victoria 3800, Australia.
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MeSH Terms
Arcuate Nucleus / cytology,  drug effects,  metabolism
Brain / cytology,  drug effects*,  metabolism
Cell Communication / drug effects
Gene Expression
In Situ Hybridization
Leptin / administration & dosage,  pharmacology*
Models, Biological
Nerve Fibers / drug effects,  metabolism,  physiology
Neurons / cytology,  metabolism,  physiology
Neuropeptide Y / genetics,  metabolism*
Preoptic Area / cytology,  drug effects,  metabolism
Pro-Opiomelanocortin / genetics,  metabolism*
Receptors, Leptin / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Suppressor Proteins / genetics,  metabolism*,  pharmacology
Reg. No./Substance:
0/Leptin; 0/Neuropeptide Y; 0/Receptors, Leptin; 0/Tumor Suppressor Proteins; 66796-54-1/Pro-Opiomelanocortin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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