| Kir6.2 knockout alters neurotransmitter release in mouse striatum: an in vivo microdialysis study. | |
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MedLine Citation:
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PMID: 18524485 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ATP-sensitive potassium (K-ATP) channels have been demonstrated to play important roles in the brain. In the present study, Kir6.2 knockout (Kir6.2-/-) mice were used to examine the contribution of Kir6.2-containing K-ATP channels to the regulation of neurotransmitter release via in vivo microdialysis studies. The results showed that the extracellular levels of monoamine and amino acid neurotransmitters in Kir6.2-/- mouse striatum were similar to those in Kir6.2+/+ mice under basal conditions. After high K+ (100mM) perfusion, the extracellular levels of DA and amino acids were increased in both genotypes. These increases, however, were significantly lower in Kir6.2-/- mice than those in Kir6.2+/+ mice. Extracellular levels of 3,4-dihydroxyphenylacetic acid (DOPAC), a major metabolite of DA, were increased in Kir6.2-/- mice but decreased in Kir6.2+/+ mice in response to high K+ stimulus. The releases of 4-hydroxy-3-methoxy-phenylacetic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were attenuated to a similar extent in both mouse genotypes. Taken together, this study provides direct in vivo evidence that Kir6.2-containing K-ATP channels play regulatory roles in neurotransmitter release in the striatum. |
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Authors:
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Xue-Ru Shi; Jing Chang; Jian-Hua Ding; Yi Fan; Xiu-Lan Sun; Gang Hu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-14 |
Journal Detail:
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Title: Neuroscience letters Volume: 439 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-16 Completed Date: 2008-09-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 230-4 Citation Subset: IM |
Affiliation:
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Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, 140 Hanzhong Road, Nanjing, Jiangsu 210029, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3,4-Dihydroxyphenylacetic Acid
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metabolism Animals Corpus Striatum / drug effects, metabolism* Dopamine / metabolism Extracellular Fluid / drug effects, metabolism Homovanillic Acid / metabolism Mice Mice, Inbred C57BL Mice, Knockout Microdialysis / methods Neurotransmitter Agents / metabolism* Potassium Channels, Inwardly Rectifying / deficiency* Potassium Chloride / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Kir6.2 channel; 0/Neurotransmitter Agents; 0/Potassium Channels, Inwardly Rectifying; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 306-08-1/Homovanillic Acid; 7447-40-7/Potassium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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