| Kir4.1 channel expression is essential for parietal cell control of acid secretion. | |
| | |
MedLine Citation:
|
PMID: 21367857 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Kir4.1 channels were found to colocalize with the H(+)/K(+)-ATPase throughout the parietal cell (PC) acid secretory cycle. This study was undertaken to explore their functional role. Acid secretory rates, electrophysiological parameters, PC ultrastructure, and gene and protein expression were determined in gastric mucosae of 7-8-day-old Kir4.1-deficient mice and WT littermates. Kir4.1(-/-) mucosa secreted significantly more acid and initiated secretion significantly faster than WT mucosa. No change in PC number but a relative up-regulation of H(+)/K(+)-ATPase gene and protein expression (but not of other PC ion transporters) was observed. Electron microscopy revealed fully fused canalicular membranes and a lack of tubulovesicles in resting state Kir4.1(-/-) PCs, suggesting that Kir4.1 ablation may also interfere with tubulovesicle endocytosis. The role of this inward rectifier in the PC apical membrane may therefore be to balance between K(+) loss via KCNQ1/KCNE2 and K(+) reabsorption by the slow turnover of the H(+)/K(+)-ATPase, with consequences for K(+) reabsorption, inhibition of acid secretion, and membrane recycling. Our results demonstrate that Kir4.1 channels are involved in the control of acid secretion and suggest that they may also affect secretory membrane recycling. |
| | |
Authors:
|
Penghong Song; Stephanie Groos; Brigitte Riederer; Zhe Feng; Anja Krabbenhöft; Michael P Manns; Adam Smolka; Susan J Hagen; Clemens Neusch; Ursula Seidler |
Related Documents
:
|
803777 - Mucosal and hepatic metabolism during the spontaneous disappearance of salicylate-induc... 21510267 - Relationship between barotolerance and fatty acids constitution of brain cell membrane. 21131367 - Il-23-dependent and -independent enhancement pathways of il-17a production by lactic acid. 1535287 - Treatment of acne vulgaris with salicylic acid pads. 7766187 - The recognition system of dietary fatty acids by the rat small intestinal cells. 12903937 - Differentiation of natural and synthetic phenylalanine and tyrosine through natural abu... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-03-02 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 286 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-04-18 Completed Date: 2011-06-28 Revised Date: 2012-04-23 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 14120-8 Citation Subset: IM |
Affiliation:
|
Department of Gastroenterology, Hepatology, and Endocrinology, Hannover Medical School, D-30625 Hannover, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Transport Electrophysiology / methods Endocytosis Gastric Acid / metabolism* Gastric Mucosa / metabolism Gene Expression Regulation* H(+)-K(+)-Exchanging ATPase / chemistry Mice Mice, Knockout Microscopy, Electron, Transmission / methods Microscopy, Fluorescence / methods Parietal Cells, Gastric / cytology* Potassium / chemistry Potassium Channels, Inwardly Rectifying / biosynthesis, physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Potassium Channels, Inwardly Rectifying; 0/potassium inwardly-rectifying channel, subfamily J, member 10; 7440-09-7/Potassium; EC 3.6.1.10/H(+)-K(+)-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cyclic AMP-dependent protein kinase regulates the alternative splicing of tau exon 10: a mechanism i...
Next Document: Toll-like receptor 3 (TLR3) induces apoptosis via death receptors and mitochondria by up-regulating ...