| Kinin-induced prostaglandin synthesis by renal papillary collecting tubule cells in culture. | |
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MedLine Citation:
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PMID: 6264808 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells having morphological and histochemical properties of collecting tubules were isolated from rabbit renal papillae. Confluent monolayer cultures of these renal papillary collecting tubule (RPCT) cells formed hemicysts and adhered with morphological asymmetry to Millipore filters. Cultures of 1-day-old RPCT cells synthesized cAMP in response to arginine vasopressin (AVP) (half-maximal response to 10(-10) M), oxytocin, and parathyroid hormone (half-maximal responses at 5 X 10(-9) M) but not to adrenergic agents. After 10 days of growth (fourfold increase in cell number) RPCT cells retained the same pattern of histochemical and hormonal responses as 1-day-old cells. Hormones were tested for their influence on the release of immunoreactive prostaglandins (iPG) by RPCT cells; the major product under both basal and stimulated conditions was iPGE2. At very low concentrations (greater than or equal to 10(-10) M), bradykinin, lysyl-bradykinin, and methionyl-lysyl-bradykinin caused four- to sixfold increases in the rate of iPGE2 formation within 3 min; smaller (less than twofold) increases were observed with relatively high concentrations of epinephrine (10(-5) M), norepinephrine (10(-5) M), and angiotensin II (10(-7) M), but only after longer incubations. Significantly, neither AVP (10(-7) M) nor [deamino]AVP (10(-7) M) caused prostaglandin release by RPCT cells. Our results indicate that kinins can act directly on the collecting tubule to elicit PGE2 formation; furthermore, this effect of kinins may be natriuretic, since PGE2 has been shown to inhibit Na+ resorption by the medullary collecting tubule and thick ascending limb. |
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Authors:
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F C Grenier; T E Rollins; W L Smith |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 241 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1981 Jul |
Date Detail:
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Created Date: 1981-08-27 Completed Date: 1981-08-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: F94-104 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects Cells, Cultured Cyclic AMP / metabolism Hormones / pharmacology Kidney Tubules / drug effects, physiology*, ultrastructure Kinins / pharmacology* Membrane Potentials Microscopy, Electron Prostaglandins / biosynthesis* Rabbits Radioimmunoassay |
| Grant Support | |
ID/Acronym/Agency:
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AM-22042/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hormones; 0/Kinins; 0/Prostaglandins; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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