Document Detail


Kinetochore fibre dynamics outside the context of the spindle during anaphase.
MedLine Citation:
PMID:  15039774     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromosomes move polewards as kinetochore fibres shorten during anaphase. Fibre dynamics and force production have been studied extensively, but little is known about these processes in the absence of the spindle matrix. Here we show that laser-microbeam-severed kinetochore fibres in the cytoplasm of grasshopper spermatocytes maintain a constant length while turning over in a polarized manner. Tubulin incorporates at or near the kinetochore and translocates towards severed ends without shortening the fibre. Consequently, the chromosome cannot move polewards unless the severed fibre reattaches to the pole through microtubules. A potential seclusion artefact has been ruled out, as fibres severed inside spindles behave identically despite being surrounded by the spindle matrix. Our data suggest that kinetochore microtubules constantly treadmill during anaphase in insect cells. Treadmilling is an intrinsic property of microtubules in the kinetochore fibre, independent of the context and attachment of the spindle. The machinery that depolymerizes minus ends of kinetochore microtubules is functional in a non-spindle context. Attachment to the pole, however, is required to cause net kinetochore fibre shortening to generate polewards forces during anaphase.
Authors:
Wei Chen; Dahong Zhang
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2004-02-22
Journal Detail:
Title:  Nature cell biology     Volume:  6     ISSN:  1465-7392     ISO Abbreviation:  Nat. Cell Biol.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-24     Completed Date:  2004-04-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100890575     Medline TA:  Nat Cell Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  227-31     Citation Subset:  IM    
Affiliation:
Department of Zoology and the Centre for Gene Research and Biotechnology, Oregon State University, 3029 Cordley Hall, Corvallis, OR 97331, USA.
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MeSH Terms
Descriptor/Qualifier:
Anaphase / physiology*
Animals
Cell Polarity / physiology
Cells, Cultured
Cytoplasm / metabolism,  ultrastructure
Grasshoppers
Kinetochores / physiology*
Lasers
Male
Microtubules / metabolism*,  ultrastructure
Mitotic Spindle Apparatus / physiology*
Protein Transport / physiology
Spermatocytes / metabolism*,  ultrastructure
Stress, Mechanical
Tubulin / metabolism
Chemical
Reg. No./Substance:
0/Tubulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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