Document Detail

Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreER(T) to assay temporal activity windows along the proximodistal limb skeleton.
MedLine Citation:
PMID:  16894608     Owner:  NLM     Status:  MEDLINE    
Cartilage differentiation occurs over a broad time range from early embryonic development, when the mesenchymal condensations that give rise to cartilage models for future bone first appear, and continuing through adult life, when there is ongoing maintenance of articular joint surfaces and re-activation of cartilage formation after fracture. The chondrogenic response also figures in the pathogenesis of degenerative and inflammatory joint diseases. We have generated a transgenic line expressing tamoxifen-dependent Cre recombinase that gives efficient recombination in the chondrogenic lineage, both during embryogenesis and postnatally, and provides a valuable tool for analysis of gene function selectively in chondrogenic cells using conditional genetic approaches. Because the cartilage model of the limb skeleton forms progressively in a proximodistal order during discrete, well-defined time periods, evaluation of the spatial extent of tamoxifen-induced recombination along the limb axis during these time windows has also enabled us to examine the pharmacokinetics of single-dose tamoxifen injections during pregnancy.
Eiichiro Nakamura; Minh-Thanh Nguyen; Susan Mackem
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  235     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-30     Completed Date:  2006-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2603-12     Citation Subset:  IM    
Copyright Information:
Copyright 2006 Wiley-Liss, Inc.
Laboratory of Pathology, Center for Cancer Research, NCI, NIH, Bethesda, Maryland 20892, USA.
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MeSH Terms
Animals, Newborn
Base Sequence
Cartilage / drug effects,  embryology*,  enzymology*,  growth & development
Chondrogenesis / drug effects
Collagen Type II / genetics
DNA Primers / genetics
Extremities / embryology*,  growth & development
Gene Expression Regulation, Developmental / drug effects
Genes, Reporter
Gestational Age
Integrases / administration & dosage,  metabolism*
Lac Operon
Mice, Transgenic
Recombination, Genetic / drug effects
Tamoxifen / pharmacokinetics,  pharmacology*
Reg. No./Substance:
0/Col2a1 protein, mouse; 0/Collagen Type II; 0/DNA Primers; 10540-29-1/Tamoxifen; EC 2.7.7.-/Cre recombinase; EC 2.7.7.-/Integrases

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