Document Detail


Kinetics of serotonin uptake in the dog lung.
MedLine Citation:
PMID:  7263447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A bolous containing [3H]serotonin and indocyanine green dye was rapidly introduced into the pulmonary artery on an isolated blood-perfused left lower dog lung lobe. Sequential samples of the venous effluent were collected for approximately 25 s following the injections. The lung intake of the injected [3H]serotonin was inversely proportional to the injected dose, ranging from about 70% for a 10-nmol injection to about 38% for a 100-nmol injection. Less than 2% of the injected 3H, which appeared in the venous effluent over the sampling interval, was in the form of [3H]5-hydroxyindoleacetic acid; and during the bolous transit through the lung lobe, uptake of serotonin by platelets was negligible. The serotonin extraction ratio curves exhibited dose-dependent characteristic shapes that we have interpreted using a model in which it is assumed that serotonin uptake follows Michaelis-Menten kinetics and flow through the capillary bed is heterogeneous. Using this model and a multiple regression analysis, the outflow dye and 3H concentration vs. time curves were used to estimate Km and Vmax for serotonin uptake by the lungs.
Authors:
D A Rickaby; J H Linehan; T A Bronikowski; C A Dawson
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology: respiratory, environmental and exercise physiology     Volume:  51     ISSN:  0161-7567     ISO Abbreviation:  J Appl Physiol Respir Environ Exerc Physiol     Publication Date:  1981 Aug 
Date Detail:
Created Date:  1981-10-25     Completed Date:  1981-10-25     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  7801242     Medline TA:  J Appl Physiol Respir Environ Exerc Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  405-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Dogs
Kinetics
Lung / metabolism*
Perfusion
Serotonin / blood,  metabolism*
Grant Support
ID/Acronym/Agency:
HL-24349/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
50-67-9/Serotonin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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