Document Detail

Kinetics of re-establishing H3K79 methylation marks in global human chromatin.
MedLine Citation:
PMID:  20699226     Owner:  NLM     Status:  MEDLINE    
We employ a stable isotope strategy wherein both histones and their methylations are labeled in synchronized human cells. This allows us to differentiate between old and new methylations on pre-existing versus newly synthesized histones. The strategy is implemented on K79 methylation in an isoform-specific manner for histones H3.1, H3.2, and H3.3. Although levels of H3.3K79 monomethylation are higher than that of H3.2/H3.1, the rate of establishing the K79 methylation is the same for all three isoforms. Surprisingly, we find that pre-existing "old" histones continue to be K79-monomethylated and -dimethylated at a rate equal to the newly synthesized histones. These observations imply that some degree of positional "scrambling" of K79 methylation occurs through the cell cycle.
Steve M M Sweet; Mingxi Li; Paul M Thomas; Kenneth R Durbin; Neil L Kelleher
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-08-09
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  285     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-18     Completed Date:  2010-11-24     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  32778-86     Citation Subset:  IM    
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
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MeSH Terms
Cell Cycle / physiology*
Chromatin / metabolism*
HeLa Cells
Histones / metabolism*
Grant Support
Reg. No./Substance:
0/Chromatin; 0/Histones

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