| Kinetics of re-establishing H3K79 methylation marks in global human chromatin. | |
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MedLine Citation:
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PMID: 20699226 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We employ a stable isotope strategy wherein both histones and their methylations are labeled in synchronized human cells. This allows us to differentiate between old and new methylations on pre-existing versus newly synthesized histones. The strategy is implemented on K79 methylation in an isoform-specific manner for histones H3.1, H3.2, and H3.3. Although levels of H3.3K79 monomethylation are higher than that of H3.2/H3.1, the rate of establishing the K79 methylation is the same for all three isoforms. Surprisingly, we find that pre-existing "old" histones continue to be K79-monomethylated and -dimethylated at a rate equal to the newly synthesized histones. These observations imply that some degree of positional "scrambling" of K79 methylation occurs through the cell cycle. |
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Authors:
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Steve M M Sweet; Mingxi Li; Paul M Thomas; Kenneth R Durbin; Neil L Kelleher |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-08-09 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 285 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-18 Completed Date: 2010-11-24 Revised Date: 2011-10-24 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 32778-86 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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physiology* Chromatin / metabolism* Hela Cells Histones / metabolism* Humans Methylation |
| Grant Support | |
ID/Acronym/Agency:
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GM 067193-08/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Histones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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