Document Detail


KINETICS OF MATING OF FP+ AND FP- STRAINS OF PSEUDOMONAS AERUGINOSA.
MedLine Citation:
PMID:  14329456     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Loutit, J. S. (University of Otago, Dunedin, N.Z.), and L. E. Pearce. Kinetics of mating of FP(+) and FP(-) strains of Pseudomonas aeruginosa. J. Bacteriol. 90:425-430. 1965.-Recently, we reported the variability of the FP(+) x FP(-) mating in Pseudomonas aeruginosa, and the increase in efficiency when the cells were mated after dilution. The present work shows that when standardized cells were mated after a dilution of 10(-2) for FP(-) and 2 x 10(-3) for FP(+), there was less variability in the system. Investigation of pairing between FP(+) and FP(-) cells, as measured by genetic transfer, revealed that none took place during 24 hr in liquid media in which growth of both parents was possible. Transfer in liquid minimal medium was not investigated, because the single marker in the male was insufficient to control back mutation in 24 hr. No transfer occurred on solid media which allowed full growth of both FP(+) and FP(-) cells. Transfer took place very slowly on minimal agar, requiring 19 to 20 hr. It is thought that this represents growth of microcolonies with eventual transfer when internal nutrients were exhausted.
Authors:
J S LOUTIT; L E PEARCE
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  90     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1965 Aug 
Date Detail:
Created Date:  1965-11-01     Completed Date:  1996-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  425-30     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Agar*
Bacteriophages*
Culture Media*
Drug Resistance, Microbial*
Genetics*
Kinetics*
Leucine*
Metabolism*
Mutation*
Pseudomonas aeruginosa*
Research*
Streptomycin*
Tryptophan*
Chemical
Reg. No./Substance:
0/Culture Media; 57-92-1/Streptomycin; 61-90-5/Leucine; 73-22-3/Tryptophan; 9002-18-0/Agar
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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