Document Detail


Kinetics of l-Valine Uptake in Suspension-Cultured Cells and Protoplast-Derived Cells of Tobacco: Comparison of Wild-Type and the Val-2 Mutant.
MedLine Citation:
PMID:  16665513     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
A kinetic analysis was made of l-valine uptake in protoplast-derived cells (mesophyll protoplasts cultured for 6 days) and in suspension-cultured cells of tobacco (Nicotiana tabacum L., cv Xanthi). Cells from wild-type and Val(r)-2 mutant plants were compared. A low-K(m) component was found in protoplast-derived cells (K(m) = 45 +/- 5 micromolar) as well as in suspension-cultured cells (K(m) = 84 +/- 21 micromolar). In the mutant cells the V(max) of this component was 12- to 14-fold less than in wild-type cells. A second component (K(m) = 2.4 +/- 0.7 millimolar) was found in suspension-cultured cells but not in protoplast-derived cells; its V(max) was the same in wild-type and mutant cells. A third component was apparently unsaturable (linear component). It was present in protoplast-derived cells but not in suspension-cultured cells, and had the same magnitude in wild-type and mutant cells. The results are discussed with reference to the uptake of l-valine in leaf tissue, in which the three kinetic components have been found simultaneously. The reduced V(max) of the low-K(m) component in the Val(r)-2 mutant, and the differential expression of the other two components in suspension-cultured cells and protoplast-derived cells indicate that the kinetically distinguishable components represent physically distinct transport systems.
Authors:
M Pilon; A C Borstlap
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  84     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  737-42     Citation Subset:  -    
Affiliation:
Botanisch Laboratorium, Rijksuniversiteit Utrecht, Lange Nieuwstraat 106, 3512 PN Utrecht, The Netherlands.
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