Document Detail


Kinetics of juvenile sea bass (Dicentrarchus labrax, L.) systemic and mucosal antibody secreting cell response to different antigens (Photobacterium damselae spp. piscicida, Vibrio anguillarum and DNP).
MedLine Citation:
PMID:  11417719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ELISPOT assay was used to measure the number of specific antibody secreting cells (ASC) induced during the primary and secondary immune responses in the spleen, head kidney and gut of juvenile (5 g) sea bass (Dicentrarchus labrax) to bacterial (Vibrio anguillarum and Photobacterium damselae ssp. piscicida) and hapten dinitrophenyl-conjugated to keyhole limpet haemocyanin (DNP-KLH) antigens administered intraperitoneally. High variability among individuals was observed at each sampling day. All fish were bath vaccinated to V. anguillarum at an earlier stage (2 g) in the farm of origin prior to the development of the experiments, and therefore only secondary and tertiary responses were measured in the group immunised with this bacterium. Significant differences to the controls were observed in the primary responses of the head kidney and the spleen to P. damselae ssp. piscicida and DNP, respectively. Frequency analysis of the production of ASC suggests that significant responses in the gut might be masked by the high error variance. The peak of the primary response was observed 4 days earlier to DNP (18-20 days post-immunisation) and it was significantly higher than the response to P. damselae ssp. piscicida. Higher numbers of ASC were observed in the secondary responses of the head kidney and spleen, although they were not statistically significantly different from the primary levels, probably due to the high error variance as supported by the frequency analysis. Nevertheless, together with a faster response (peak at 7 days post-immunisation), the data suggest that memory formation had occurred. Additionally, the data suggest that some suppression of the secondary immune response in the gut might have occurred. The head kidney appears to produce the highest number of specific ASC of the organs tested. It appears that sea bass show a relatively fast but short duration antibody response.
Authors:
N M dos Santos; J J Taverne-Thiele; A C Barnes; A E Ellis; J H Rombout
Related Documents :
1092609 - Effect of corynebacterium parvum on the class and subclass of antibody produced in the ...
6984419 - Antibody formation in mouse bone marrow during secondary type responses to various thym...
9272209 - Effect of boar seminal immunosuppressive component on humoral immune response in mice.
163279 - Modulation of in vivo antibody responses by cholera toxin.
25212389 - Synthesis of tumor-associated muc1-glycopeptides and their multivalent presentation by ...
22380759 - Allo-exposure status and leucocyte antibody positivity of blood donors show a similar r...
1765089 - Molecular and cellular aspects of immunologic tolerance.
21062789 - Development of retinol-binding protein 4 immunocolloidal gold fast test strip using hig...
9488159 - Complement-mediated cytolysis and azidothymidine are synergistic in hiv-1 suppression.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Fish & shellfish immunology     Volume:  11     ISSN:  1050-4648     ISO Abbreviation:  Fish Shellfish Immunol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-06-21     Completed Date:  2001-12-04     Revised Date:  2013-11-14    
Medline Journal Info:
Nlm Unique ID:  9505220     Medline TA:  Fish Shellfish Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  317-31     Citation Subset:  IM    
Affiliation:
Institute for Molecular and Cell Biology, University of Porto, Portugal.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibody-Producing Cells / drug effects*,  immunology
Antigens, Bacterial / pharmacology*
Bass / immunology*
Dinitrobenzenes / pharmacology
Enzyme-Linked Immunosorbent Assay / veterinary
Hemocyanin / pharmacology
Intestinal Mucosa / cytology,  immunology
Kidney / cytology,  immunology
Leukocytes / drug effects,  immunology
Photobacterium / immunology
Spleen / cytology,  immunology
Vibrio / immunology
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Dinitrobenzenes; 9013-72-3/Hemocyanin; FV4Y0JO2CX/keyhole-limpet hemocyanin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Immunomodulatory effects of dietary intake of chitin on gilthead seabream (Sparus aurata L.) innate ...
Next Document:  The kinetics of the hypoferraemic response and changes in levels of alternative complement activity ...