Document Detail


Kinetics of iodide uptake and efflux in various human thyroid cancer cells by expressing sodium iodide symporter gene via a recombinant adenovirus.
MedLine Citation:
PMID:  12792733     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We evaluated the potential of radioiodide therapy in human sodium iodide symporter (hNIS)-defective thyroid cancer cells via exogenous hNIS expression. Three human thyroid cancer cells (ARO, FRO and NPA) of different origin were transduced by a recombinant adenovirus encoding hNIS expression cassette (Rad-hNIS). The cells were efficiently transduced by a recombinant adenovirus in a virus dose-dependent manner. Consequently, the hNIS protein could be readily detected by Western blot analysis 48-h post-infection at 10 infectious virus particles per cell. These hNIS-transduced cells actively transported iodide into the cytoplasm at the level of 11635.3, 61571.6, and 19367.5 pmoles/10(6) cells in ARO, FRO, and NPA, respectively. However, a significant amount of iodide was eluted to an iodide-free media within 60 min in all the cell lines. RT-PCR analysis revealed that the expression of genes related to iodide trapping (Tg, TSHR and TPO) was dramatically downregulated in these cells. The present study indicates that functional hNIS can be efficiently expressed and is responsible for active transport of iodide in hNIS-negative human thyroid cancer cells by a recombinant adenovirus. However, the human thyroid cancer cells, along with downregulation of iodide metabolism-related gene expression, lose the ability to maintain iodide. Therefore, these kinetic characteristics of iodide uptake and efflux may limit the therapeutic potential of hNIS/radioiodide-based treatment following exogenous hNIS expression in human thyroid cancer.
Authors:
Won Woo Lee; Boyoung Lee; Sung Jin Kim; Jungsun Jin; Dae Hyuk Moon; Heuiran Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  10     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:    2003 Jul-Aug
Date Detail:
Created Date:  2003-06-06     Completed Date:  2004-02-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  845-9     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Ulsan College of Medicine, Songpa, PO Box 145, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics*
DNA, Recombinant
Defective Viruses
Gene Expression*
Humans
Iodide Peroxidase / genetics,  metabolism
Iodine Radioisotopes / diagnostic use,  pharmacokinetics
Kinetics
RNA, Messenger / analysis
Receptors, Thyrotropin / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sodium Iodide / pharmacokinetics*
Symporters / genetics*,  metabolism
Thyroid Neoplasms / metabolism*
Transfection
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/DNA, Recombinant; 0/Iodine Radioisotopes; 0/RNA, Messenger; 0/Receptors, Thyrotropin; 0/Symporters; 0/sodium-iodide symporter; 7681-82-5/Sodium Iodide; EC 1.11.1.8/Iodide Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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