Document Detail


Kinetics of intramuscular triglyceride fatty acids in exercising humans.
MedLine Citation:
PMID:  11053362     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A pulse ([(14)C]palmitate)-chase ([(3)H]palmitate) approach was used to study intramuscular triglyceride (imTG) fatty acid and plasma free fatty acid (FFA) kinetics during exercise at approximately 45% peak O(2) consumption in 12 adults. Vastus lateralis muscle was biopsied before and after 90 min of bicycle exercise; (3)H(2)O production, breath (14)CO(2) excretion and lipid oxidation (indirect calorimetry) rates were measured during exercise. Results: during exercise, 8.2+/-1.2 and 8.4+/-0.7 micromol x kg(-1) x min(-1) of imTG fatty acids and plasma FFA, respectively, were oxidized according to isotopic measurements. The sum of these two values was not different (P = 0.6) from lipid oxidation by indirect calorimetry (15.4 +/-1.6 micromol x kg(-1) x min(-1)); the isotopic and indirect calorimetry values were correlated (r = 0.79, P<0.005). During exercise, imTG turnover rate was 0.32+/-0.07%/min (6.0+/-2.0 micromol of imTG x kg wet muscle(-1) x min(-1)) and plasma FFA were incorporated into imTG at a rate of 0.7+/-0.1 micromol x kg wet muscle(-1) x min(-1). The imTG pool size did not change during exercise. This pulse-chase, dual tracer appears to be a reasonable approach to measure oxidation and synthesis kinetics of imTG.
Authors:
Z Guo; B Burguera; M D Jensen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  89     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-11-17     Completed Date:  2001-01-04     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2057-64     Citation Subset:  IM    
Affiliation:
Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota 55905, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Calorimetry, Indirect
Carbon Radioisotopes / diagnostic use
Energy Metabolism / physiology
Exercise / physiology*
Fatty Acids, Nonesterified / blood,  diagnostic use,  pharmacokinetics*
Female
Humans
Leg
Male
Muscle, Skeletal / metabolism*
Oxidation-Reduction
Palmitic Acid / diagnostic use,  pharmacokinetics
Postural Balance
Triglycerides / blood,  diagnostic use,  pharmacokinetics*
Tritium / diagnostic use
Grant Support
ID/Acronym/Agency:
DK-40484/DK/NIDDK NIH HHS; DK-45343/DK/NIDDK NIH HHS; RR-0585/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fatty Acids, Nonesterified; 0/Triglycerides; 10028-17-8/Tritium; 57-10-3/Palmitic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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