Document Detail

Kinetics of the intracellular availability of heme after supplementing a heme-deficient yeast mutant with 5-aminolevulinate.
MedLine Citation:
PMID:  3017377     Owner:  NLM     Status:  MEDLINE    
The 5-aminolevulinic acid synthetase-deficient yeast mutant ole3 (Bard, M., Woods, R.A. & Haslam, J.M. (1974) Biochem. Biophys. Res. Commun. 56, 324-330), pregrown in a 5-aminolevulinic acid containing medium, can grow on glucose or galactose medium in the absence of heme for about 13 generations. When supplemented with 5-aminolevulinate before their 9th cell division, the cells can be induced to full respiratory competence. From the measurement of heme-dependent parameters (e.g. respiration, transcription of iso-1-cytochrome c mRNA and the post-translational proteolytic processing of the heme-free intermediate precursor of cytochrome c1 to its heme-containing mature form) it can be judged that heme is available in the cell about one hour after the addition of 5-aminolevulinate. The onset of respiration, however, does not occur to an appreciable extent before the 3rd hour of induction. The heme analogue deuteroporphyrin IX prevents respiratory adaptation but yet effects the transcription of heme-controlled genes.
W Schmalix; U Oechsner; V Magdolen; W Bandlow
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological chemistry Hoppe-Seyler     Volume:  367     ISSN:  0177-3593     ISO Abbreviation:  Biol. Chem. Hoppe-Seyler     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-09-30     Completed Date:  1986-09-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503054     Medline TA:  Biol Chem Hoppe Seyler     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  379-85     Citation Subset:  IM    
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MeSH Terms
Aminolevulinic Acid / metabolism*
Cytochrome c Group / genetics
Cytochromes / metabolism
Cytochromes c*
Cytoplasm / metabolism
Gene Expression Regulation
Heme / biosynthesis*,  physiology
Levulinic Acids / metabolism*
Oxygen Consumption
Protein Processing, Post-Translational
RNA, Messenger / genetics
Saccharomyces cerevisiae / genetics,  metabolism*,  ultrastructure
Saccharomyces cerevisiae Proteins*
Time Factors
Transcription, Genetic
Reg. No./Substance:
0/CYC1 protein, S cerevisiae; 0/Cytochrome c Group; 0/Cytochromes; 0/Levulinic Acids; 0/RNA, Messenger; 0/Saccharomyces cerevisiae Proteins; 106-60-5/Aminolevulinic Acid; 14875-96-8/Heme; 9007-43-6/Cytochromes c

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