| Kinetics of the intracellular availability of heme after supplementing a heme-deficient yeast mutant with 5-aminolevulinate. | |
| | |
MedLine Citation:
|
PMID: 3017377 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The 5-aminolevulinic acid synthetase-deficient yeast mutant ole3 (Bard, M., Woods, R.A. & Haslam, J.M. (1974) Biochem. Biophys. Res. Commun. 56, 324-330), pregrown in a 5-aminolevulinic acid containing medium, can grow on glucose or galactose medium in the absence of heme for about 13 generations. When supplemented with 5-aminolevulinate before their 9th cell division, the cells can be induced to full respiratory competence. From the measurement of heme-dependent parameters (e.g. respiration, transcription of iso-1-cytochrome c mRNA and the post-translational proteolytic processing of the heme-free intermediate precursor of cytochrome c1 to its heme-containing mature form) it can be judged that heme is available in the cell about one hour after the addition of 5-aminolevulinate. The onset of respiration, however, does not occur to an appreciable extent before the 3rd hour of induction. The heme analogue deuteroporphyrin IX prevents respiratory adaptation but yet effects the transcription of heme-controlled genes. |
| | |
Authors:
|
W Schmalix; U Oechsner; V Magdolen; W Bandlow |
Related Documents
:
|
1913997 - New hypocholesterolemic abietamide derivatives. ii. synthesis and hypocholesterolemic a... 17517337 - Functional characterization of a desaturase from the tobacco hornworm moth (manduca sex... 8365407 - The solution structure of the histidine-containing protein (hpr) from staphylococcus au... 1794897 - Regulation of cadmium induced porphyria by ascorbic acid in chick embryos. 12802527 - Biosynthesis of fructo-oligosaccharides by sporotrichum thermophile during submerged ba... 23258397 - Seafood: nutritional benefits and risk aspects. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biological chemistry Hoppe-Seyler Volume: 367 ISSN: 0177-3593 ISO Abbreviation: Biol. Chem. Hoppe-Seyler Publication Date: 1986 May |
Date Detail:
|
Created Date: 1986-09-30 Completed Date: 1986-09-30 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8503054 Medline TA: Biol Chem Hoppe Seyler Country: GERMANY, WEST |
Other Details:
|
Languages: eng Pagination: 379-85 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aminolevulinic Acid
/
metabolism* Cytochrome c Group / genetics Cytochromes / metabolism Cytochromes c* Cytoplasm / metabolism Gene Expression Regulation Heme / biosynthesis*, physiology Levulinic Acids / metabolism* Oxygen Consumption Protein Processing, Post-Translational RNA, Messenger / genetics Saccharomyces cerevisiae / genetics, metabolism*, ultrastructure Saccharomyces cerevisiae Proteins* Time Factors Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
|
0/CYC1 protein, S cerevisiae; 0/Cytochrome c Group; 0/Cytochromes; 0/Levulinic Acids; 0/RNA, Messenger; 0/Saccharomyces cerevisiae Proteins; 106-60-5/Aminolevulinic Acid; 14875-96-8/Heme; 9007-43-6/Cytochromes c |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Chemical dependencies of learning in the rabbit olfactory bulb: acquisition of the transient spatial...
Next Document: The influence of forskolin on the metabolism of ecdysone and 20-hydroxyecdysone in isolated fat body...